2004 Fiscal Year Final Research Report Summary
Development of model animal to reveal the antibody production for ABO blood group antigens.
| Project/Area Number |
15590586
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Jichi Medical School |
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Principal Investigator |
IWAMOTO Sadahiko Jichi Medical School, Dept.of Medicine, Professor, 医学部, 教授 (10232711)
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| Co-Investigator(Kenkyū-buntansha) |
KAMESAKI Toyomi Jichi Medical School, Dept.of Medicine, Lecturer, 医学部, 講師 (90316513)
OKUDA Hiroshi Jichi Medical School, Dept.of Medicine, Assos.Professor, 医学部, 助教授 (50285772)
KUMADA Maki Jichi Medical School, Dept.of Medicine, Assist.Professor, 医学部, 助手 (40326830)
OMI Toshinori Jichi Medical School, Dept.of Medicine, Assist.Professor, 医学部, 助手 (40296091)
FUKUI Emiko Utsunomiya University, Faculty of Agriculture, Assist.Professor, 農学部, 助教授 (20208341)
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| Project Period (FY) |
2003 – 2004
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| Keywords | ABO transgenic rat / transplantation / ABO evolution / rat ABO antigen / 移植抗原 |
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| Research Abstract |
Histo-blood group ABH antigens are important not only for blood transfusion but also for organ transplantation. To improve the clinical management of transplantation, we have established transgenic rats expressing human A-transferase and B-transferase gene. Wild Wistar rats expressed A-antigen in salivary gland, intestine, and urinary bladder tissue, but B-antigen was not stained in any organs studied. A-transgenic rats expressed A-antigen in ectopic tissue of the brain plexus, type II lung epithelium, pancreas, and epidermis. B-antigen in the B-transgenic rat was expressed in the same organs as A-transgenic rats. When small intestines of wild Wistar were transplanted into A- or B-trangenic rats, the grafts were rapidly rejected compared to the grafts from A- or B-transgenic rats into wild. The systemic expression of A- or B-antigens may have modified the terminal organization of sugar chain antenna and have stimulated the innate-immune system. We have cloned authologous genes of ABO transferase from Wistar rat, which consisted of three A-transferase and one B-transferase genes. These cDNAs were mapped on rats genome draft sequence. Rat chromosome 1 accommodates B-transferase gene and chromosome 3 encodes tandem arrayed three A-transferase copies. These facts indicated that the A- and B-transferase genes were established as paralogous genes in some mammals. Primates encode single ABO gene. If the ABO ancestral genes consisted of multicopy genes with A and B transferase activity like rats, they must donate or accept gene fragments from each other and may produce a locus composed of A-alleles and B-alleles. The multicopy genes may possibly gather and bundle into a single gene through unequal cross over. This estimation must be resolved by further genome projects in other mammals.
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Research Products
(13 results)
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[Journal Article] A novel mutation in the RHD gene in Japanese individuals with weak D, encoding an amino acid change in the 11th transmembranous domain of the RhD protein.2003
Author(s)
Kamesaki T, Kumada M, Omi T, Okuda H, Iwamoto S, Takahashi J, Kimura K, Hirayama F. Kamata H, Obara K, Taniguchi M, Tani Y, Kajii E.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Aly/REF, a factor for mRNA transport, activates RH gene promoter function
Author(s)
Hiroshi Suganuma, Maki Kumada, Toshinori Omi, Takaya Gotoh, Munkhtulga Lkhagvasuren, Hiroshi Okuda, Toyomi Kamesaki, Eiji Kajii, Sadahiko Iwamoto
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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