2005 Fiscal Year Final Research Report Summary
Role of class II MHC bound antigenic peptide in the pathogenesis of inflammatory bowel disease.
| Project/Area Number |
15590676
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Osaka City University |
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Principal Investigator |
OSHITANI Nobuhide Osaka City University, Graduate School of Medicine, Associate Professor, 医学研究科, 助教授 (20231235)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Kenji Osaka City University, Graduate School of Medicine, Hospital Lecturer, 医学研究科, 病院講師 (70382041)
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| Project Period (FY) |
2003 – 2005
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| Keywords | Inflammatory bowel disease / Crohn's disease / ulcerative colitis / class II MHC / antigenic peptide / tandem mass analysis / amino acid sequence |
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| Research Abstract |
Isolation of antigenic peptides from the MHC-groove has contributed to the understanding of T cell immune responses. We already reported antigenic peptides isolated from human intestinal HLA-DR grove contained various enteral antigens.
However, naturally bound peptide ligands for HLA-DP have yet to be reported in clinical samples. We examined antigenic peptides bound to the HLA-DP as well as HLR-DR groove in human intestine by ion-trap tandem mass spectrometry equipped with online reverse-phase high performance liquid chromatography. We detected 7 HLA-DR associated peptides and 10 HLA-DP associated peptides from 4 controls, 4 patients with ulcerative colitis, and 9 patients with Crohn's disease. The calculated molecular masses (m/z) of these peptides reangesd from 1430.7 to 2987.3 and 1212.6 to 2105.0 representing 15 to 26 and 15 to 18 amino-acid residues from HLA-DR and HLA-DP associated peptides, respectively. Most of these parent proteins were exogenous proteins including Escherichia coli, and Carnorhabditis elegans. The present results suggest that in vivo antigen processing by antigen-presenting cells and T lymphocytes in human intestine are participated with exogenous antigen presentation.
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Research Products
(10 results)
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[Journal Article] Significance of increased proliferation of immature plasma cells in the appendix of patients with ulcerative colitlis.2005
Author(s)
Kawachiya T, Oshitani N, Jinno Y, Watanabe K, Nakamura S, Fujiwara Y, Higuchi K, Maeda K, Nishiguchi Y, Hirakawa K, Matsumoto T, Arakawa T.
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Journal Title
Int J Mol Med 15
Pages: 417-423
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Mycobacterial trehalose 6, 6' -dimycolate preferentially induces type 1 helper T cell responses through signal transducer and activator of transcription 4 protein.2005
Author(s)
Oiso R, Fujiwara N, Yamagami H, Maeda S, Matsumoto S, Nakamura S, Oshitani N, Matsumoto T, Arakawa T, Kobayashi K
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Journal Title
MicrobPathogenesis 39
Pages: 35-43
Description
「研究成果報告書概要(和文)」より
-
-
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-
[Journal Article] Significance of increased proliferation of immature plasma cells in the appendix of patients with ulcerative colitis.2005
Author(s)
Kawachiya T, Oshitani N, Jinno Y, Watanabe K, Nakamura S, Fujiwara Y, Higuchi K, Maeda K, Nishiguchi Y, Hirakawa K, Matsumoto T, Arakawa T
-
Journal Title
Int J Mol Med 15
Pages: 417-423
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Mycobacterial trehalose 6,6'-dimycolate preferentially induces type 1 helper T cell responses through signal transducer and activator of transcription 4 protein.2005
Author(s)
Oiso R, Fujiwara N, Yamagami H, Maeda S, Matsumoto S, Nakamura S, Oshitani N, Matsumoto T, Arakawa T, Kobayashi K
-
Journal Title
MicrobPathogenesis 39
Pages: 35-43
Description
「研究成果報告書概要(欧文)」より
-
-
