2004 Fiscal Year Final Research Report Summary
Three-dimensional microstructural abnormality of the coronary capillary network after myocardial reperfusion- A Three-Dimensional Confocal Laser Microscopic Study -
| Project/Area Number |
15590779
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
WATANABE Nozomi Kawasaki Medical School, Cardiology, Assistant Professor, 医学部, 講師 (60319960)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIMOTO Katsukuni Kawasaki Medical School, Anatomy, Associate Professor, 医学部, 助教授 (80106351)
AKASAKA Takashi Kawasaki Medical School, Cardiology, Associate Professor, 医学部, 助教授 (70322584)
YOSHIDA Kiyoshi Kawasaki Medical School, Cardiology, Professor, 医学部, 教授 (60322583)
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| Project Period (FY) |
2003 – 2004
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| Keywords | reperfusion injury / Nicorandil |
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| Research Abstract |
Background. Previous experimental and clinical studies have reported that Nicorandil, an adenosine triphosphate (ATP)-sensitive K^+ channel opener, exerts beneficial effect on microvascular function and clinical outcome after myocardial ischemia. We assessed whether intravenous administration of Nicorandil affect capillary three-dimensional (3-D) architectural characteristics and capillary volume, after early myocardial reperfusion.
Methods and Results. Using opened-chest anesthetized rats' hearts, the left anterior descending artery was occluded for 7 min followed by reperfusion. Nicorandil or saline was infused intravenously during occlusion and reperfusion. Entire coronary microvasculature was filled with contrast medium, after the hearts were arrested. Capillaries were observed 3-dimensionally, in control area and reperfused area in all samples utilizing confocal laser scanning microscopy. Capillary volume fraction was computed from 3-D images. Reperfused area in both nicorandil and saline groups showed characteristic architectural changes in capillaries. In nicorandil group, capillary volume fraction in the reperfused area was significantly reduced in saline group, compared with nicorandil group [12.7±7.2% versus 18.1±5.3% (p<0.01)].
Conclusions. Intravenous nicorandil administration beneficially affects capillary damages.
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Research Products
(4 results)
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[Journal Article] Effect of Nicorandil, K^+_<ATP>-channel Opener, on Coronary Capillary, Architecture and Volume After Early Myocardial Ischemia-Reperfusion : - A Three-Dimensional Confocal Laser Microscopic Study -2004
Author(s)
Watanabe N, Akasaka T, Fujimoto K, Kaita T, Shigeto F, Ogasawara Y, Neishi T, Yoshida K.
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Journal Title
Circulation Journal 68(12)
Pages: 1210-1214
Description
「研究成果報告書概要(和文)」より
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