2004 Fiscal Year Final Research Report Summary
Gene therapy for Fabry mice
| Project/Area Number |
15590844
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Niigata University |
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Principal Investigator |
MARUYAMA Hiroki Niigata University, Medical and Dental Hospital, Assistant, 医歯学総合病院, 助手 (10293218)
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| Project Period (FY) |
2003 – 2004
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| Keywords | naked DNA / CAG promoter / Fabry disease / α-galactosidase / hydrodynamic-based transfection / kidney-targeted gene transfer / renal vein / catheter |
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| Research Abstract |
We recently developed a novel kidney-targeted gene transfer technique, using the retrograde renal vein injection of naked plasmid DNA and indicated that the kidney serves as a depot organ for the production of therapeutic proteins. Fabry disease is an X-linked recessive inborn metabolic disorder characterized by systemic and vascular accumulation of globotriaosylceramide (Gb3) caused by a deficiency of the lysosomal enzyme α-galactosidase A (α-Gal A). Gene therapy is expected to revolutionize the treatment of Fabry disease. We examined the α-Gal A transfer to the treatment of mice with α-Gal A knock out (Fabry mice). Naked plasmid DNA expressing human α-Gal A (pKSCX-α-Gal A) solution was rapidly injected into the left kidneys via the retrograde renal vein. pKSCX solution was similarly injected into control mice (pKSCX mice).
We confirmed the presence of vector-derived α-Gal A mRNA in the left kidneys by reverse transcriptase polymerase chain reaction and observed plasma α-Gal A levels in pKSCX-α-Gal A-injected mice. Compared with the pKSCX mice, the pKSCX-α-Gal A mice showed significant therapeutic effects: increase of α-Gal A in injected kidney, liver, heart, and decrease of Gb3 in heart, bilateral kidneys, liver, spleen confirmed by thin-layer chromatography analysis.
These results demonstrate that kidney-targeted pKSCX-α-Gal A gene transfer by retrograde renal vein injection reduces systemic accumulation of Gb3 in Fabry mice.
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Research Products
(33 results)
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[Journal Article] A novel method to assay proteins in blood plasma after intravenous injection of plasmid DNA.2004
Author(s)
H.Hanawa, R.Watanabe, M.Hayashi, T.Yoshida, S.Abe, S.Komura, H.Liu, R.Elnaggar, H.Chang, Y.Okura, K.Kato, M.Kodama, H.Maruyama, J.Miyazaki, Y.Aizawa
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Journal Title
Tohoku.J.Exp.Med. 202
Pages: 155-161
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Post-secretion neutralization of transgene-derived effect : Soluble erythropoietin receptor/IgG_1Fc expressed in liver neutralizes erythropoietin produced in muscle.2004
Author(s)
H.Maruyama, M.Higuchi, N.Higuchi, S.Kameda, M.Saito, M.Sugawa, J.Matsuzaki, T.Neichi, S.Yokoyama, Y.Miyazaki, J.Miyazaki, F.Gejyo
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Journal Title
J.Gene Med. 6
Pages: 228-237
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Antagonist of monocyte chemoattractant protein 1(CCL2) prevents the initiation and progression of lupus nephritis and renal vasculitis in MRL/lpr mice.2003
Author(s)
H.Hasegawa, M.Kohno, M.Sasaki, A.Inoue, M.R.Ito, M.Terada, K.Hieshima, H.Maruyama, J.Miyazaki, O.Yoshie, M.Nose, S.Fujita
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Journal Title
Arthritis Rheum. 48
Pages: 2555-2566
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Coxsackievirus and adenovirus receptor(CAR)-positive immature osteoblasts as targets of adenovirus-mediated gene transfer for fracture healing.2003
Author(s)
T.Ito, K.Tokunaga, H.Maruyama, H.Kawashima, H.Kitahara, T.Horikoshi, A.Ogose, Y.Hotta, R.Kuwano, H.Katagiri, N.Endo
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Journal Title
Gene Ther. 10
Pages: 1623-1628
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Hydrodynamics-based delivery of the viral interleukin-10 gene suppresses experimental crescentic glomerulonephritis in Wistar-Kyoto rats.2003
Author(s)
N.Higuchi, H.Maruyama, T.Kuroda, S.Kameda, N.Iino, H.Kawachi, Y.Nishikawa, H.Hanawa, H.Tahara, J.Miyazaki, F.Gejyo
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Journal Title
Gene Ther. 10
Pages: 1297-1310
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Sustained transgene expression in rat kidney using naked plasmid DNA and PCR-amplified DNA fragments.
Author(s)
H.Maruyama.N.Higuchi, S.Kameda, G.Nakamura, M.Shimotori, N.Iino, M.Higuchi, T.Neichi, S.Yokoyama, T.Kono, J.Miyazaki, F.Gejyo
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Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Effect of hydrodynamics-based gene delivery of plasmid DNA encoding IL-1 receptor antagonist-Ig for treatment of rat autoimmune myocarditis : Possible mechanism for lymphocytes and non-cardiac cells.
Author(s)
H.Liu, H.Hanawa, T.Yoshida, R.Elnaggar, M.Hayashi, R.Watanabe, K.Toba, K.Yoshida, H.Chang, Y.Okura, K.Kato, M.Kodama, H.Maruyama, J.Miyazaki, M.Nakazawa, Y.Aizawa
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Journal Title
Description
「研究成果報告書概要(欧文)」より
