2004 Fiscal Year Final Research Report Summary
Mithochondrial pH in virtal inslinoma cell line INS-1 with glucose stimulant Ca release
| Project/Area Number |
15590957
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
OGATA Makiko Tokyo Women's Medical University, Physiology, 医学部, 助手 (10233404)
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| Co-Investigator(Kenkyū-buntansha) |
AWAJI Takeo Tokyo Women's Medical University, Physiology, assistant professor, 医学部, 講師 (60297546)
IWASAKI Naoko Tokyo Women's Medical University, Physiology, assistant professor, 医学部, 講師 (70203370)
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| Project Period (FY) |
2003 – 2004
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| Keywords | insulin secreting cell / green fluorescent protein / Ca^<2+> oscillations / β細胞 / MODY |
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| Research Abstract |
The adjacent pH of the mithochondrial cytochrome IV conjugated with pH sensor green fluorescent protein (Mit-AfpH, Mit-V/FpH) were simultaneously assessed using real-time imaging of living, stably expressed CHO-K1 cells and INS-1 cells.
Mit-AFpH and Mt-VFpH fluorescence was detected in a cytosol and show mithochondrial formation. Stably expressed CHO-K1 cells and INS-1 cells added CCCP showed dropping of pH and surfaced by digitoninn show integrant change according to extracellular pH changing. Even in the Glucose free condition, INS-1 show slightly lower pH than CHO-K1 cells. And gllucose stimulation made no change of mitochondrial pH in CHO-K1 cells but decreasing more from the baseline in INS-1 cells toward Ca oscillation.
These results shows insulin secreting cells were continuously activating mitochondria at base condition and more activating by glucose existence. Normally, insulin shows continuous secreting in base line. Although INS-1 is the insulinoma cell line, the insulin secreting reaction may be more active but simultaneous as normal β cells reaction in glucose stimulation. This stable cell line is the unique and newly tool for mitoghondrial function study using vital cells.
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Research Products
(4 results)
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[Journal Article] Genetic variants in the caplan-10 gene and the development of type 2 diabetes in the Japanese population.2005
Author(s)
Naoko Iwasaki, Yukio Horikawsa, Takafumi Tsuchiya, Yutaka Kitamura, Takahiro Nakamura, Yukio Tanizawa, Makiko Ogata, Naoyuki Kamatani, yasuhiko Iwamoto, Laura del Bosque-Plata, M.Geoffrey Hayes, Nancy J.Cox, Graeme I.Bell
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Journal Title
J.Hum.Genet. 50
Pages: 92-98
Description
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