2005 Fiscal Year Final Research Report Summary
虚血再潅流障害における腸管粘膜再生に対する骨髄由来細胞の役割:実験的検討
| Project/Area Number |
15591361
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Juntendo University |
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Principal Investigator |
YANAI Toshihiro Juntendo University, School of Med., Department of Pediatric General and Urogenital Surgery, Assistant Professor, 医学部, 講師 (20317481)
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| Co-Investigator(Kenkyū-buntansha) |
KATO Yoshifumu Juntendo University, School of Med., Department of Pediatric General and Urogenital Surgery, Asssistant Professor, 医学部, 講師 (60286739)
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| Project Period (FY) |
2003 – 2005
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| Keywords | ischemia-reperfusion injury / mouse / Bone marrow cell / Green fluorescence protein / Small intestine |
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| Research Abstract |
Previous studies in our laboratories have shown that fetal small intestinal graft can survive and develop after transplantation in recipient tissue without any vascular reconstruction. However, whether adult small intestinal graft can survive or not and the mechanisms by which these intestinal grafts regenerate and develop are still unclear.
We examined whether adult small bowel grafts (ASBGs) can survive transplantation without vascular reconstruction if graft serosectomy (SS) is performed. Our results suggest that adult small bowel can be transplanted successfully in rats without vascular reconstruction by removing the graft serosa. Furthermore, SS allows vascular endothelial growth factor (VEGF) mRNA and, subsequently, VEGF protein in ASBG to be induced very soon after transplantation, which may contribute to the survival of ASBG transplanted without vascular reconstruction.
We also studied that if granulocyte colonystimulating factor (G-CSF) or basic fibroblast growth factor (bFGF) could improve graft survival in the same rat model and the results suggest that G-CSF and bFGF enhance angiogenesis and mucosal surface expansion of the ASBG.
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Research Products
(4 results)
