2005 Fiscal Year Final Research Report Summary
Experimental study how to reduce necrotic change of ischemic injured intestine by acute visceral artery occlusion.
| Project/Area Number |
15591482
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
KUNIYOSHI Yukio University of the Ryukyus, Faculty of medicine, Professor, 医学部, 教授 (50153317)
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| Co-Investigator(Kenkyū-buntansha) |
ARAKAKI Katsuya University of the Ryukyus, University Hospital, Instructor, 医学部附属病院, 助手 (90325853)
MIYAGI Kazufumi University of the Ryukyus, Faculty of medicine, Instructor, 医学部, 助手 (00229808)
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| Project Period (FY) |
2003 – 2005
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| Keywords | visceral ischemia / bowel viability |
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| Research Abstract |
Objects : To clarify the time limitation after starting mesenteric ischemia whether the injured intestine should be extirpated, we investigated the intestinal change in a dog model by observing the following items ; 1)intestinal regional peristalsis, 2)electric potential of intestine muscle, 3)intestinal intramural oxygenation, and 4)microscopically change of the intestinal wall.
Method : The cranial artery of dogs was snared. By occlusion of the artery by ligating the snare, a model was established, and the 4 items were measured at interval of 1,6, and 12 hours after arterial occlusion. The peristalsis was observed by measuring the intraluminal pressure by the tonometer placed in the intestine. The intestinal electrical muscle activity was measured by two electrodes placed in intestinal muscle. After the measurement of these variables, the specimen of intestine was excised, and prepared for microscopical evaluation.
Result : By recirculation of intestine, 1)in 1 hour ischemia model, the former 3 items normalized within an hour. There was no microscopical ischemic change in the whole range of the intestine. 2)In 6 hours ischemia model, 30% of the intestine was changed to be necrotic, but the remaining of the affected intestine had intact crypt. The former 3 items normalized at the normal segment of intestine. 3)In 12 hours ischemia model, there was no sign of viability of intestine.
Conclusion, in this dog models, mesenteric ischemia for 6 hours and over induces irreversible intestinal change which should be extirpated.
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