2004 Fiscal Year Final Research Report Summary

Gale therapy for osteoarthritis

Research Project

Project/Area Number	15591585
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Orthopaedic surgery
Research Institution	Ehime University
Principal Investigator	WATANABE Shohei Ehime University, School of Medicine, Associate Professor, 医学部, 助教授 (80253300)
Project Period (FY)	2003 – 2004
Keywords	Gene therapy / Osteoarthritis / Adenovirus / SRT / ort mice / IL-4
Research Abstract	1.To determine the therapeutic effect of IL-4 mediated adenovirus vector on the murine osteoarthritis model, AdCIL4 was administrated to the male 16-week-old SRT/ort mice intravenously. It was observed that knee joints form the mice to which injected with AdCIL4 showed significantly inhibited to affect with osteoarthritic changes compared with those of control mice. Although there was the problem to induce the inflammation by AdCIL4, it seemed that local administration with vectors could be a therapeutic method for osteochondral lesion. 2.To examine the effect of IL-4 on matrix synthesis in chondrocytes under excessive mechanical stress in vitro. Mechanical stress for 16 hours significantly decreased levels of mRNA for both AGG and CII (p<0.01), but rat IL-4 at doses of 1 or 10 ng/ml recovered these levels (p<0.05). These findings suggested that IL-4 might prevent articular cartilage from damage caused by mechanical stress and therefore could be a useful therapeutic agent for treatment of arthritic disorders.

(4 results)