2004 Fiscal Year Final Research Report Summary
Analysis of Ewing sarcoma using a transgenic mouse
| Project/Area Number |
15591593
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
YOSHIDA Koichi School of Medicine, Biology, Professor, 医学部, 教授 (60117653)
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| Co-Investigator(Kenkyū-buntansha) |
WADA Takuro School of Medicine, Orthopedic Surgery, Associate Professor, 医学部, 助教授 (00244369)
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| Project Period (FY) |
2003 – 2004
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| Keywords | Ewing sarcoma / mouse model of disease / fusion oncogene / EWS-FLI / cre-loxP / chromosome translocation / telomerase / ETS transcription factor |
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| Research Abstract |
A goal of this study is to express the oncogene in mother tissue for Ewing sarcoma-genesis and to isolate a model laboratory mouse. Furthermore, it is to identify a target gene which the EWS-FLI fusion transcription factor controls. We isolated a transgenic mouse of two strains carrying EWS-FLI oncogene. These mice had reproductive potential and were able to propagate in a normal condition. We crossbred these mice with the laboratory mouse which expresses Cre enzyme in neural crest. This allows us to induce a recombination of DNA in vivo and an activation of EWS-FLI oncogene in neural crest-derived cells. Since mouse was not obtained in enough numbers, we were unable to judge sarcoma-genesis. We are going to isolate a different strain of transgenic mouse and crossbreed it with the Cre transgenic mouse again. In the cell line which we introduced the oncogene EWS-FLI into, we found the gene which expression was significantly altered. We made clear by cDNA array method that the tenascin gene, an extra-cellular matrix component, was activated by EWS-FLI. Also we found that a fusion transcription factor activated telomerase gene which is associated with cellular immortalization. We continue manufacture of model laboratory mouse of Ewing sarcoma and are going to examine a role of target gene in development of the neoplasm.
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Research Products
(12 results)
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[Journal Article] EWS/ETS Fusions activate telomerase in Ewing's tumors2003
Author(s)
Takahashi A, Higashino F, Aoyagi M, Yoshida K, Itoh M, Kyo S, Ohno T, Taira T, Ariga H, Nakajima K, Hatta M, Kobayashi M, Sano H, Kohgo T, Shindo M.
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Journal Title
Cancer Res 63
Pages: 8338-8344
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A tetraspanin-family protein, T-cell acute lymphoblastic leuhemia-associated antigen, is induced by the Ewing's sarcoma-Wilms'tumor 1 fusion protein of desmoplastic small round cell tumor2003
Author(s)
Ito E, Honma R, Imai J-I, Azuma S, Kanno T, Mori S, Yoshie O, Nishio J, Iwasaki H, Yoshida K, Gohda J, Inoue J-I, Watanabe S, Semba K.
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Journal Title
Am J Pathol 163
Pages: 2165-2172
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Induction of Tenascin-C by Tumor specific EWS-ETS fusion genes2003
Author(s)
Watanabe G, Nishimori H, Irifune H, Sasaki Y, Ishida S, Zenbutsu H, Tanaka T, Kawaguchi S, Wada T, Hata J, Kusakabe M, Yoshida K, Nakamura Y, Tokino T.
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Journal Title
Genes Chromosomes and Cancer 36
Pages: 224-232
Description
「研究成果報告書概要(欧文)」より
