2004 Fiscal Year Final Research Report Summary
signal transduction of epithelial cells in response to secretagogues
| Project/Area Number |
15591806
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Mie University |
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Principal Investigator |
KISHIOKA Chikako Mie University, Faculty of Medicine, Research Associate, 医学部, 助手 (10314113)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEUCHI Kazuhiko Mie University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (50206942)
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| Project Period (FY) |
2003 – 2004
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| Keywords | mucin / secretion / signal transduction |
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| Research Abstract |
Chronic obstructive airway disease is marked by excess production of airway mucus. The molecular mechanisms underlying this pathology are unknown. ATP and PAF are detected in inflamed airways, and suggested to contribute to mucus overproduction. We examined the possibility that ATP and PAF contributes to mucus hypersecretion. Result in cultured cells showed that MUC5AC expression increased in response to ATP and PAF. These appeared to be mediated by intracellular pathways involving JNK/PKA/CREB for ATP and MAPK/JNK for PAF. (We used reporter assay and specific inhibitors.) Such identification of pathways involved in MUC5AC up-regulation by ATP and PAF in the HM3 cells may serve as a model for the effects of cytokines and mediators, which regulate MUC5AC expression during the airway inflammation.
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