2004 Fiscal Year Final Research Report Summary
Functional Regeneration of Inner Ear Hair Cells
| Project/Area Number |
15591809
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
FUJINO Kiyohiro KYOTO UNIVERSITY, Graduate School of medicine, Assistant Professor, 医学研究科, 助手 (50359832)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAGAWA Takayuki KYOTO UNIVERSITY, Graduate School of medicine, Assistant Professor, 医学研究科, 助手 (50335270)
TSUJI Jun KYOTO UNIVERSITY, Graduate School of medicine, Lecturer, 医学研究科, 講師 (30252448)
KITA Tomoko KYOTO UNIVERSITY, Graduate School of medicine, Researcher, 医学研究科, 研究員(科学技術振興) (20362519)
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| Project Period (FY) |
2003 – 2004
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| Keywords | Notch signaling / RBP-J / Cre-loxP / Wnt signaling / GSK-3β / hair cells |
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| Research Abstract |
1.Knockout of RBP-J
Notch signaling is the important signal transduction pathway for cell proliferation and differentiation. Inhibition of this system enables the unusual cell proliferation that lead to the regeneration of hair cell. Using adenovirus vector and Cre-loxP system in the culture preparation of organ of Corti, we knocked out the gene of RBP-J : a transcription factor of Notch signaling. We achieved the ectopic generation of hair cells.
2.Pharmacological inhibition of RBP-J
In the culture preparation of organ of Corti, RBP-J was blocked by γ-secretase. Similarly to the genetic inhibition of RBP-J, ectopic hair cells were generated.
3.Knockout of GSK-3β
Wnt signaling is the important signal transduction pathway for cell proliferation. Activating Wnt signaling enable abnormal cell proliferation. We knocked out GSK-3β ; a inhibiting factor of Wnt signaling with the similar method to the RBP-J. We found the ectpic hair cell growth.
4.Pharmacological inhibition of GSK-3β
In the culture preparation of organ of Corti, GSK-3β was blocked by several drugs. Similarly to the genetic inhibition of RBP-J, ectopic hair cells were generated.
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Research Products
(38 results)
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[Journal Article] Expression of beta-catenin in developing auditory epithelia of mice.2004
Author(s)
Takebayashi S, Nakagawa T, KoJima K, Kim TS, Kita T, Dong Y, Endo T, Iguchi F, Naito Y, Omori K, Ito J.
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Journal Title
Acta Otolaryngol Suppl 551
Pages: 18-21
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Expression of calretinin by fetal otocyst cells after transplantation into damaged rat utricle explants.2004
Author(s)
Kim TS, KoJima K, Nishida AT, Tashiro K, Lee JE, FuJino K, Nakagawa T, Naito Y, Omori K, Lefebvre P, Ito J.
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Journal Title
Acta Otolaryngol Suppl 551
Pages: 34-38
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Surgical techniques for cell transplantation into the mouse cochlea2004
Author(s)
Iguchi F, Nakagawa T, Tateya I, Endo T, Kim TS, Dong Y, Kita T, KoJima K, Naito Y, Omori K, Ito J.
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Journal Title
Acta Otolaryngol Suppl 551
Pages: 43-47
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Transplantation of neural stem cells into the modiolus of mouse cochleae injured by cisplatin.2004
Author(s)
Tamura T, Nakagawa T, Iguchi F, Tateya I, Endo T, Kim TS, Dong Y, Kita T, KoJima K, Naito Y, Omori K, Ito J.
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Journal Title
Acta Otolaryngol Suppl 551
Pages: 65-68
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Transplantation of bone marrow stromal cells into the cochlea of chinchillas.2004
Author(s)
Naito Y, Nakamura T, Nakagawa T, Iguchi F, Endo T, FuJino K, Kim TS, Hiratsuka Y, Tamura T, Kanemaru S, Shimizu Y, Ito J.
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Journal Title
Neuroreport 15
Pages: 1-4
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Expression of beta-catenin in developing auditory epithelia of mice.2004
Author(s)
Takebayashi S, Nakagawa T, Kojima K, Kim TS, Kita T, Dong Y, Endo T, Iguchi F, Naito Y, Omori K, Ito J.
-
Journal Title
Acta Otolaryngol Suppl
Pages: 18-21
Description
「研究成果報告書概要(欧文)」より
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-
-
[Journal Article] Expression of calretinin by fetal otocyst cells after transplantation into damaged rat utricle explants.2004
Author(s)
Kim TS, Kojima K, Nishida AT, Tashiro K, Lee JE, Fujino K, Nakagawa T, Naito Y, Omori K, Lefebvre P, Ito J.
-
Journal Title
Acta Otolaryngol Suppl
Pages: 34-38
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Surgical techniques for cell transplantation into the mouse cochlea.2004
Author(s)
Iguchi F, Nakagawa T, Tateya I, Endo T, Kim TS, Dong Y, Kita T, Kojima K, Naito Y, Omori K, Ito J.
-
Journal Title
Acta Otolaryngol Suppl
Pages: 43-47
Description
「研究成果報告書概要(欧文)」より
-
-
[Journal Article] Transplantation of neural stem cells into the modiolus of mouse cochleae injured by cisplatin.2004
Author(s)
Tamura T, Nakagawa T, Iguchi F, Tateya I, Endo T, Kim TS, Dong Y, Kita T, Kojima K, Naito Y, Omori K, Ito J.
-
Journal Title
Acta Otolaryngol Suppl
Pages: 65-68
Description
「研究成果報告書概要(欧文)」より
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-
-
-
[Journal Article] Transplantation of bone marrow stromal cells into the cochlea of chinchillas.2004
Author(s)
Naito Y, Nakamura T, Nakagawa T, Iguchi F, Endo T, Fujino K, Kim TS, Hiratsuka Y, Tamura T, Kanemaru S, Shimizu Y, Ito J.
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Journal Title
Neuroreport 15
Pages: 1-4
Description
「研究成果報告書概要(欧文)」より
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