Estimation of cancer susceptibility and risk of head and neck carcinoma by gene polymorphism and expression.

2004 Fiscal Year Final Research Report Summary

• Project/Area Number: 15591826
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: NAKAI Shigeru Kyoto Prefectural Univ, of Medicine, Otolaryngology-Head and Neck Surgery, Lecturer, 医学研究科, 講師 (30295654)
• Co-Investigator(Kenkyū-buntansha): BANBA Hitoshi Kyoto Prefectural Univ, of Medicine, Otolaryngology-Head and Neck Surgery, Associate, 医学研究科, 助手 (30360035) ; SHIMADA Taketoshi Kyoto Prefectural Univ, of Medicine, Otolaryngology-Head and Neck Surgery, Associate, 医学研究科, 助手 (30275226) ; HAMA Takemitsu Kyoto Prefectural Univ, of Medicine, Otolaryngology-Head and Neck Surgery, Associate, 医学研究科, 助手 (90315953)
• Project Period (FY): 2003 – 2004
• Keywords: DNA repair gene / XPD / ERCC2 gene / XRCC1 gene / laryngeal carcinoma / gene polymorphism

Research Abstract

Genetic differences in the ability to metabolize carcinogens, repair DNA damage and induce apoptosis may contribute to the variation in cancer risk in population at large. The capacity of DNA repair genes may a play important role in carcinogenesis with Tabacco- related cancer. We hypothesize that inherited polymorphisms of XPD/ERCC2 exson 23 and XRCC1 codon 26304 may contribute to genetic susceptibility to SCCHN. These polymorphisms were investigated with PCR-RFLP method. The result of XPD/ERCC2 exson 23 polymorphisms are below, control : wild home type 27 cases (93.1%), hetero type 2 cases (6.9%), mutant type 0 case (0%), patient (laryngeal carcinoma ; SCC) : wild home type 54 cases (91.5%), hetero type 5 cases (8.5%), mutant type 0 case (0%). The result of XRCC1 codon 26304 polymorphisms are below, control : wild home type 25 cases (86.2%), hetero type 4 cases (13.8%), mutant type 0 case (0%), patient : wild home type 52 cases (88.1%), hetero type 6 cases (10.2%), mutant type 1 case (1.7%). There were no signicicant between XPD/ERCC2, XRCC1 polymorphisms and susceptibility to laryngeal carcinoma. We concluded that these polymorphisms are not indicator of susceptibility to Tabacco- related cancer.