An experimental study for temporomandibular joint arthritis using with ultrasound gene induction

2004 Fiscal Year Final Research Report Summary

• Project/Area Number: 15592126
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Kyushu Dental College
• Principal Investigator: TOMINAGA Kazuhiro Kyushu Dental College, Dept.of Oral and Maxillofacial Surg, Associate Professor, 歯学部, 助教授 (40188793)
• Co-Investigator(Kenkyū-buntansha): NISHIHARA Tatsuji Kyushu Dental College, Dept.of Health Promotion, Professor, 歯学部, 教授 (80192251)
• Project Period (FY): 2003 – 2004
• Keywords: temporomandibular joint / arthritis / gene induction / ultrasound / animal experiment

Research Abstract

Interleukin-1 (IL-1) plays dominant roles in pathogenesis of arthritic conditions in many joints including the temporomandibular joint. Administration of exogenous IL-1 receptor antagonist (IL-1ra) is found to be effective in reducing inflammatory response mediated by IL-1. Snce administration of cytokine itself is very costly and works only for short period, gene induction of this kind of antagonists is thought to be worthy. Recently, nonviral gene induction, which is thought to be safer than viral gene induction, has been developed The aim of this study was to evaluate the efficacy of IL-1ra gene induction in adjuvant-induced arthritis. Nineteen Lewis rats were divided into three groups, i.e., control, arthritis with human IL-1ra gene induction and arthritis with empty plasmid induction. Arthritis was induced with conventional manner. In vivo sonoporation was performed 4 times ; 14 days and 7 days prior to injection of adjuvant, the day and 7 days after injection of adjuvant. The efficacy of the gene induction was evaluated clinically with hind paw swelling following development of arthritis. Histopathological evaluation was done on day 17 post-adjuvant injection with a published scoring system. The onset of arthritis in the IL-1ra induction group was delayed comparing with the empty plasmid group. On day 17 post-adjuvant injection, the volume of the hind paw increased 0.26 0.03 ml and 0.76 0.09 ml in the IL-1ra induction group and in the empty plasmid group, respectively. The swelling of hind paw was significantly reduced by IL-1ra induction with sonoporation. The arthritic scores in the IL-1ra induction group were significantly lower than those of the empty plasmid group. As conclusions in vivo IL-1ra gene induction is effective far reduction of arthritis in adjuvant induced arthritis.

Research Products

• [Journal Article] Relationship between TNF-αandTUNEL-positive chondrocytes in antigen-induced arthritis of the rabbit temporomandibular joint. (2006)
  • Author(s): Hirota, Y., Habu, M., Tominaga, K., Sukedai, M., Matsukawa, A., Nishihara, T.,Fukuda,J.
  • Journal Title: J Oral Pathol Med 35 Pages: 91-8
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Relationship between TNF-α and TUNEL-positive chondrocytes in antigen-induced arthritis of the rabbit temporomandibular joint. (2006)
  • Author(s): Hirota, Y., Habu, M., Tominaga, K., Sukedai, M., Matsukawa, A., Nishihara, T., Fukuda, J.
  • Journal Title: J Oral Pathol Med 35 Pages: 91-98
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Involvement of tumor necrosis factor-α and interleukin-8 in antigen-induced arthritis of the rabbit temporomandibular joint. (2004)
  • Author(s): Sukedai, M.Tominaga, K.Habu, M., Masukawa, A., Nishihara, T.and Fukuda,J.
  • Journal Title: J Oral Pathol Med 33 Pages: 102-10
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A decrease in the molecular weight of hyaluronic acid in synovial fluid from patients with temporomandibular disorders. (2004)
  • Author(s): Takahashi, T., Tominaga, K., Takano, H., Ariyoshi, W., Habu, M., Fukuda, J.and Maeda,H.
  • Journal Title: J Oral Pathol Med 33 Pages: 224-9
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Detection and significance of the characteritic magnetic signals of mandibular condyles in children. (2004)
  • Author(s): Morimoto, Y., Tominaga, K., Konoo, T., Tanaka, T., and Ohba,T.
  • Journal Title: Oral Surg Oral Med Oral Pathol 97 Pages: 269-75
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] IL-1beta,IL-1 receptor antagonist and soluble type IIIL-1 receptor in synovial fluid of patients with temporomandibular disorders (2004)
  • Author(s): Tominaga, K., Habu, M., Sukedai, M., Hirota, Y., Takahashi, T.and Fukuda,J
  • Journal Title: Arch Oral Biol 49 Pages: 493-9
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Alternation of the magnetic resonance signals characteristic of madibular condyles during rrowth. (2004)
  • Author(s): Morimoto, Y., Tominaga, K., Konoo, T., Tanaka, T., Yamaguchi, K., Fukuda, J.and Ohba,T.
  • Journal Title: Oral Surg Oral Med Oral Pathol Oral Rabiol Endod 98 Pages: 348-54
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Delivery of cytolethal distending toxin B induces cell cycle arrest and apoptosis in gingival squamous cell carcinoma in vitro. (2004)
  • Author(s): Yamamoto, K., Tominaga, K., Sukedai, M., Okinaga, T., Iwanaga, K., Nishihara., Fukuda,J.
  • Journal Title: Eur J Oral Sci. 112 Pages: 445-51
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Involvement of tumor necrosis factor-α and interleukin-8 in antigen-induced arthritis of the rabbit temporomandibular joint. (2004)
  • Author(s): Sukedai, M., Tominaga, K., Habu, M., Matsukawa, A., Nishihara, T., Fukuda, J.
  • Journal Title: J Oral Pathol Med 33 Pages: 102-110
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A decrease in the molecular weight of hyaluronic acid in synovial fluid from patients with temporomandibular disorders. (2004)
  • Author(s): Takahashi, T., Tominaga, K., Takano, H., Ariyoshi, W., Habu, M., Fukuda, J., Maeda, H.
  • Journal Title: J Oral Pathol Med 33 Pages: 224-229
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Detection and significance of the characteristic magnetic signals of mandibular condyles in children. (2004)
  • Author(s): Morimoto, Y., Tominaga, K., Konoo, T., Tanaka, T., Ohba, T.
  • Journal Title: Oral Surg Oral Med Oral Pathol 97 Pages: 269-275
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] IL-1beta, IL-1 receptor antagonist and soluble type II IL-1 receptor in synovial fluid of patients with temporomandibular disorders (2004)
  • Author(s): Tominaga, K., Habu, M., Sukedai, M., Hirota, Y., Takahashi, T., Fukuda, J.
  • Journal Title: Arch Oral Biol 49 Pages: 493-499
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Alternation of the magnetic resonance signals characteristic of mandibular condyles during growth. (2004)
  • Author(s): Morimoto, Y., Tominaga, K., Konoo, T., Tanaka, T., Yamaguchi, K., Fukuda, J., Ohba, T.
  • Journal Title: Oral Surg Oral Med Oral Pathol 98 Pages: 348-354
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Delivery of cytolethal distending toxin B induces cell cycle arrest and apoptosis in gingival squamous cell carcinoma in vitro. (2004)
  • Author(s): Yamamoto, K., Tominaga, K., Sukedai, M., Okinaga, T., Iwanaga, K., Nishihara, T., Fukuda, J.
  • Journal Title: Eur J Oral Sci 112 Pages: 445-451
  • Description: 「研究成果報告書概要(欧文)」より