重症ＲＳウイルス患者における危険因子および分子遺伝子型研究

2016 Fiscal Year Annual Research Report

• Project/Area Number: 15F15116
• Research Institution: Nagasaki University
• Principal Investigator: 吉田 レイミント 長崎大学, 熱帯医学研究所, 教授 (90432963)
• Co-Investigator(Kenkyū-buntansha): LE MINH NHAT 長崎大学, 熱帯医学研究所, 外国人特別研究員
• Project Period (FY): 2015-04-24 – 2017-03-31
• Keywords: RSV / pneumonia / ON1 / Vietnam

Outline of Annual Research Achievements

To investigate the clinical relevance of RSV-A ON1,acute respiratory infection (ARI) cases enrolled to our population-based prospective pediatric ARI surveillance at Khanh Hoa General Hospital, Central Vietnam from January 2010 through December 2012 were studied. Clinical-epidemiological information and nasopharyngeal samples were collected. Multiplex PCR assays were performed for screening 13 respiratory viruses. RSVpositive samples were further tested for subgroups (A/B) and genotypes information by sequencing the G-glycoprotein 2nd hypervariable region. Statistical analysis was performed to evaluate the clinicalepidemiological characteristics of RSV-A ON1. The study demonstrated the clinical relevance of RSV-A ON1 infected ARI cases. Respiratory viruses were detected in 66.2% of the cases, in which RSV (n = 426, 23.0%), Influenza (12.0%) and Rhinovirus (25.1%) were the leading respiratory viruses detected. RSV-positive cases were significantly younger, wheezing (52.8%), tachypnea (31.0%), crackle (21.6%) and chest-wall indrawing (9.9%) were significantly more common. In this study, 123 RSV-A ON1 and 138 NA1 were genotyped. ON1 cases were younger, frequently associated with underlying medical condition and respiratory sign and symptoms such as wheeze (81.3%), tachypnea (50.4%) and difficulty in breathing (18.7%). In addition, ARI cases with LRTI (34.2%) and radiologically-confirmed pneumonia (19.5%) were more commonly seen in ON1 ARI cases.

Research Progress Status

28年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

28年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] NIHE/Khanh Hoa General Hospital(ベトナム)
  • Country Name: VIET NAM
  • Counterpart Institution: NIHE/Khanh Hoa General Hospital
• [Journal Article] Association of RSV-A ON1 genotype with Increased Pediatric Acute Lower Respiratory Tract Infection in Vietnam. (2016)
  • Author(s): Yoshihara K, Le MN, Okamoto M, Wadagni AC, Nguyen HA, Toizumi M, Pham E, Suzuki M, Nguyen AT, Oshitani H, Ariyoshi K, Moriuchi H, Hashizume M, Dang DA, Yoshida LM*.
  • Journal Title: Scientific Reports Volume: 6 Pages: 27856
  • DOI: 10.1038/srep27856.
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Molecular evolution of respiratory syncytial virus subgroup A genotype NA1 and ON1 attachment glycoprotein (G) gene in central Vietnam. (2016)
  • Author(s): Yoshihara K, Le MN, Nagasawa K, Tsukagoshi H, Nguyen HA, Toizumi M, Moriuchi H, Hashizume M, Ariyoshi K, Dang DA, Kimura H, Yoshida LM*
  • Journal Title: Infect Genet Evol Volume: 45 Pages: 437-446
  • DOI: 10.1016/j.meegid.2016.10.010.
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant