2015 Fiscal Year Annual Research Report
１神経細胞における機能的ジーンサイレンシングの可視化
| Project/Area Number |
15F15711
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| Research Institution | Kyoto University |
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Principal Investigator |
影山 龍一郎 京都大学, 物質-細胞統合システム拠点, 教授 (80224369)
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| Co-Investigator(Kenkyū-buntansha) |
GOLDIE BELINDA 京都大学, 物質-細胞統合システム拠点, 外国人特別研究員
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| Project Period (FY) |
2015-07-29 – 2017-03-31
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| Keywords | Neurobiology / RNA dynamics / synaptic plasticity / learning and memory |
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| Outline of Annual Research Achievements |
(1) Establishment of single-molecule imaging of microRNA (miRNA FISH) technique in the host laboratory. (2) Creation of a functional dual-fluorescent backbone vector construct for in-vitro live-cell imaging of miRNA function. (3) Establishment of differentiation and depolarization methods for culturing human neuronal cells in the host lab. (4) Use of (3) to undertake next-generation sequencing analysis of m6A RNA modification dynamics in response to neuronal activity.
Since last May, we have been able to establish several new techniques in the laboratory, as well as refine some techniques already in place. In return, the main member of this project has learned new techniques, including the operation of new pieces of equipment not available to her in her previous institute. She has been able to make a positive contribution to the projects of others working in the group, as well as providing leadership and English language support to the more junior members. In particular, through contributing as an advisor to the project of a PhD student, she has been able to combine techniques new to the lab with those already in place to create a project of great novelty and at the forefront of the field of research. She is looking forward to being able to present this work at an international conference at the end of June. Additionally, she has been able to establish a new protocol in the lab that will enable her to add stronger evidence to a study that was part of her PhD in Australia, and that will enhance the manuscript already written and improve the impact factor of its publication.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
In parallel to the research proposal, a new interest developed in the host lab has led her to become involved in a new project, derived from her contribution to a project already in progress within the lab, which has already yielded enticing results and will continue to develop over the remainder of the fellowship.
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| Strategy for Future Research Activity |
The data analysis from the initial phase of the new project has led to the development of several hypotheses that will be tested over the coming months. She will have more concrete data prior to her presentation at the RNA conference at the end of June. Based on current progress, it will be possible to have a manuscript from this work submitted by the end of 2016.
For the original project, she is currently working to overcome the technical barriers associated with live-cell imaging in the particular neuroblastoma cell line. There are still several avenues that she has not explored and this testing is running in parallel with experiments from the new project. New equipment and reagents will continue to be tested until either a solution is reached, or this is a deficit with the cell line that needs to be documented. All of the other preliminary work for this project has already been completed, and if we are able to solve the problem we will be able to make rapid progress on the remainder of the project.
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Research Products
(2 results)
