Construction of carbon nanowall sheet edge electronics and differentiation induction control of single cell

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H02032
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plasma electronics
• Research Institution: Nagoya University
• Principal Investigator: Hori Masaru 名古屋大学, 工学研究科, 教授 (80242824)
• Co-Investigator(Kenkyū-buntansha): 近藤 博基 名古屋大学, 工学(系)研究科(研究院), 准教授 (50345930)
• Co-Investigator(Renkei-kenkyūsha): ISHIKAWA Kenji 名古屋大学, 大学院工学研究科, 特任教授 (60417384) ; TAKEDA Keigo 名城大学, 理工学部, 准教授 (00377863) ; TANAKA Hiromasa 名古屋大学, 大学院工学研究科, 特任准教授 (00508129)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: プラズマ / カーボンナノウォール / グラフェンエッジ / 分化誘導 / 骨芽細胞 / 細胞外カルシウム / 細胞内カルシウム

Outline of Final Research Achievements

In order to cultivate biotechnology devices and plasma bioelectronics that can control adhesion, morphology and differentiation induction of cells by systematic elucidation interaction between electronic properties developed from nanosheet-edges in carbon nanowall (CNW) and cells, the effects of superimposed electrical stimulation on the CNW scaffold on the proliferation / differentiation (ossification) of osteoblasts were elucidated. Proliferation promotion and suppression of ossification were specifically confirmed only when the electrical stimulation at the frequency of 10 Hz was superimposed. Furthermore, the dependence on wall density and the formation of cell clumps without ossification were also found. These suggest changes in cell proliferation and differentiation due to the synergy between the sheet edge on the CNW surface and electrical stimulation, and establishment of new cell control technology in various regenerative medicine is expected.

Free Research Field

プラズマ科学