2017 Fiscal Year Final Research Report
Study of genome maintenance / inheritance mechanism based on chromosome 4D structure analysis
| Project/Area Number |
15H02369
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
須谷 尚史 東京大学, 分子細胞生物学研究所, 講師 (30401524)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 染色体高次構造 / 細胞周期 / コンデンシン / コヒーシン / 機械学習 |
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| Outline of Final Research Achievements |
For this study, we have introduced in situ Hi-C method to explore 3D and 4D structure of genome. Omics analysis pipeline that integrates Hi-C, ChIP-seq, Trasncriptome, and Genome sequence data has been developed and help us to visualize chromosome functional structures at various level. Using this pipeline, the transition of the high order structure of chromosome during cell cycle was captured. Notable achievements include 1) Condensin is important for re-annealing of single-stranded DNA generated during transcription, which activity is important for chromosome condensation, b) Two cohesin acetyltransferases (ESCO 1 and 2) in human function in different pathways. 1 induces sisterchromatids cohesion throughout the cell cycle, while 2 travels on chromosomes together with DNA helicase during replication, and this interaction with helicase prevent degradation of Esco2. c) Origin of DNA replication of the fission yeast genome is determined by three factors as revealed by machine learning.
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| Free Research Field |
ゲノム機能構造
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