2017 Fiscal Year Final Research Report
Molecular basis for cell entry of canine distemper virus
| Project/Area Number |
15H02384
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
福原 秀雄 北海道大学, 薬学研究院, 特任助教 (80707191)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 構造解析 / ウイルス / 細胞侵入 / 細胞表面受容体 / 相互作用解析 / X線結晶構造解析 / 膜融合 / ワクチン |
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| Outline of Final Research Achievements |
The structural features of the membrane fusion protein (CDV-F) of canine distemper virus (CDV) were observed by cryo-electron microscopy. The sample preparation and grid manufacturing conditions were successfully optimized for high-resolution analysis. On the other hand, for the receptor-binding protein (CDV-H) of the same virus, several anti-CDV-H monoclonal antibodies were setablished by rat intestinal lymph node method. The subtypes and CDV-H binding properties of the obtained antibodies were examined. The membrane fusion assay showed that each antibody has different binding affinity and specificity. These results give insight on the molecular basis of viral entry and vaccine effectiveness of CDV and related viruses.
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| Free Research Field |
構造生物学
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