Analysis of transition process at the molecular level of dissolved organic carbon in terrestrial area by utilizing special pulse NMR method

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H02805
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental dynamic analysis
• Research Institution: Kobe University
• Principal Investigator: FUJITAKE NOBUHIDE 神戸大学, 農学研究科, 教授 (50243332)
• Co-Investigator(Kenkyū-buntansha): 近藤 美由紀 国立研究開発法人国立環境研究所, 環境計測研究センター, 研究員 (30467211) ; 杉山 裕子 岡山理科大学, 理学部, 准教授 (40305694) ; 内田 雅己 国立極地研究所, 国際北極環境研究センター, 准教授 (70370096)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 溶存有機物 / 1H NMR / 北極湿原 / 炭素循環

Outline of Final Research Achievements

Application of 1H NMR analysis using a special pulse sequence (SPR-W5-WATERGATE) was examined on dissolved organic carbon (DOC) which is the key of the carbon cycle process in the terrestrial ecosystem. Optimization of measurement parameters in the sequence, guarantee of quantitative, and examination of pretreatment method were carried out.
As a result, it was found that a good spectrum can be obtained by measuring 0.5 to 24 hours with a sample amount of 1 mL to 50 mL even for test water having a low DOC concentration. Through application examples of DOCs taken from a wetland in the high latitude Arctic region, the utility of this method was demonstrated.

Free Research Field

土壌学