Mecahistic analysis of a protective response of skin tissue to UV genotoxicity

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H02815
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Tohoku University
• Principal Investigator: IKEHATA Hironobu 東北大学, 医学系研究科, 講師 (90250737)
• Co-Investigator(Kenkyū-buntansha): 鈴木 隆史 東北大学, 医学系研究科, 講師 (70508308)
• Co-Investigator(Renkei-kenkyūsha): MORITA Masanobu 東北大学, 医学系研究科, 助教 (10519094) ; YAMAMOTO Masayuki 東北大学, 医学系研究科, 教授 (50166823)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 紫外線 / 皮膚 / MIS応答 / アポトーシス / 突然変異 / DNA損傷 / 遺伝子組換えマウス / Nrf2

Outline of Final Research Achievements

We studied the mechanism of the response of mutation induction suppression (MIS), a protective response of skin tissue to UV genotoxicity, using a mechanistic model based on the Keap1-Nrf2 system, which controls antioxidative responses in mammalian cells. We performed an analysis of global gene expression changes in the MIS response and detected the induction of genes related to apoptosis and cell proliferation, which suggests that MIS is based on a tissue turnover mechanism with apoptosis and hyperplasia. The analysis has also suggested that the Keap1-Nrf2 system functions in the tissue recovery process from the skin damage produced after UV exposures. We also estimated the amounts of UV-induced DNA damage necessary for the MIS induction.

Free Research Field

紫外線生物影響