ベクターコントロールが及ぼす希少野生動物への影響評価と新規感受性評価法の開発

2015 Fiscal Year Annual Research Report

• Project/Area Number: 15H02825
• Research Institution: Hokkaido University
• Principal Investigator: 池中 良徳 北海道大学, (連合)獣医学研究科, 准教授 (40543509)
• Co-Investigator(Kenkyū-buntansha): 石塚 真由美 北海道大学, (連合)獣医学研究科, 教授 (50332474) ; 水川 葉月 北海道大学, (連合)獣医学研究科, 助教 (60612661) ; 中山 翔太 北海道大学, (連合)獣医学研究科, 助教 (90647629)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: Vector Control / DDT / 極新鮮肝Bank / iHep / ADME / 野生動物 / 感受性評価

Outline of Annual Research Achievements

Vector Controlのため、時に環境基準や野生動物への影響を度外視した散布もしばしば行われている。ヒト健康を尊守するうえで必要な処置であるが、この薬剤散布がどのような環境影響、野生動物への影響、しいてはヒトへの影響があるのか、評価する必要がある。一方、Vector controlには、DDTのみでなく、同時に様々なピレスロイド系農薬が使用されるが、この共散布が希少生態系にどのような影響を与えるのか、未だ報告はほとんど無い。更に、genome情報が乏しく、in vivo実験が困難な希少野生動物にこれら薬剤がどのような影響を与えるか、それを評価するための方法も未だ確立されていない。以上の背景の基、当該研究では「Vector controlで使用される薬剤のヒトおよび希少野生動物への毒性評価法を開発し、中・長期使用時の曝露riskを予測する」事を目的とする。
2015年度は、計画通り①極新鮮肝Bankの構築、②誘導性肝臓様細胞（iHep）を用いた感受性評価法の開発、③家禽（ニワトリ）および魚類（アフリカナマズ）を用いたin vivo投与試験を実施した。
①極新鮮肝Bankの構築では、猛禽類を含む希少鳥類約60サンプル、ネコ科動物を含む哺乳類約30サンプルの採取を行った。②iHepの構築では、ハイエナの皮膚線維芽細胞からiHepの誘導を試みた結果、アルブミンやE-cadherinの発現が確認出来る等、誘導に成功し始めている。③の暴露実験では、投与実験は終了し、オミクスデータを用いたシステムバイオロジー解析を進めている。

Current Status of Research Progress

1: Research has progressed more than it was originally planned.

Reason

極新鮮肝バンクの構築は、釧路市野生動物医学研究所および札幌市円山動物園の協力により、当初の目標以上に試料が集まっている。今後、採取した試料を用い、トキシコカイネティックス解析を行い、化学物質感受性評価を行う予定である。また、今年度は、初めて野生動物からのiHepの誘導に成功した点は、本研究計画において大きな進捗であると考えている。今後、ハイエナだけでは無く、他の野生動物の細胞も用い、誘導を試みる予定である。

Strategy for Future Research Activity

① Vector Control地域におけるフィールドサーベイランス:協力機関の研究者らと共同で、環境試料（水・土壌・大気）、希少野生動物（無脊椎動物・魚類・両生類・鳥類・哺乳類）の採集を行う。得られた試料は化学分析を行い、DDT濃度を明らかにすると共に、高感度バイオマーカーを解析するためマイクロアレイを中心としたオミクス解析を実施する。
②　希少野生動物の感受性評価:薬物動態解析に用いられる“ＡＤＭＥ：Ａｂsorption, Distribution, Metabolism, Excretion”の各種factorを算出することがin vivo実験が困難な希少野生動物において化学物質感受性を評価するうえで重要と考え、それぞれの項目についてin vitroもしくはin silicoによる実験でデータを取得することを試みる。また、引き続きハイエナだけでなく、他の野生動物から採取した皮膚線維芽細胞を用い、iHepへの誘導を試みる。また、得られたiHepの機能を解析すると共に、各種薬物代謝酵素群のファーマコカイネティックス解析および代謝経路の解析を行う。

Research Products

• [Int'l Joint Research] ヨハネスブルグ大学/ノースウェスト大学(南アフリカ)
  • Country Name: SOUTH AFRICA
  • Counterpart Institution: ヨハネスブルグ大学/ノースウェスト大学
• [Int'l Joint Research] ゴンダール大学/アワサ大学(エチオピア)
  • Country Name: ETHIOPIA
  • Counterpart Institution: ゴンダール大学/アワサ大学
• [Int'l Joint Research] ザガジグ大学(エジプト)
  • Country Name: EGYPT
  • Counterpart Institution: ザガジグ大学
• [Int'l Joint Research] カセサート大学(タイ)
  • Country Name: THAILAND
  • Counterpart Institution: カセサート大学
• [Journal Article] Bioaccumulation and human health risk assessment of DDT and other organochlorine pesticides in an apex aquatic predator from a premier conservation area. (2016)
  • Author(s): Gerber R, Smit NJ, Van Vuren JH, Nakayama SMM, Yohannes YB, Ikenaka Y, Ishizuka M, Wepener V.
  • Journal Title: Sci. Total. Environ. Volume: 550 Pages: 522-533
  • DOI: 10.1016/j.scitotenv.2016.01.129
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Distribution and health risk assessment of organochlorine pesticides (OCP) residue in edible cattle tissues from northeastern part of Egypt: High accumulation level of OCP in tongue (2016)
  • Author(s): Mahmoud AFA, IkenakaY, YohannesYB, DarwishWS, Eldaly EA, Morshdy AE, Nakayama SMM, MizukawaH, Ishizuka M
  • Journal Title: Chemosphere Volume: 144 Pages: 1365-1371
  • DOI: 10.1016/j.chemosphere.2015.10.016
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] β-carotene and retinol contents in the meat of herbivorous ungulates with a special reference to their public health importance (2016)
  • Author(s): Darwish WS, Ikenaka Y, Morshdy AE, Eldesoky KI, Nakayama SMM, MizukawaH, Ishizuka M
  • Journal Title: JVMS Volume: 78 Pages: 351-354
  • DOI: 10.1292/jvms.15-0287
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Accumulation properties of inorganic mercury and organic mercury in the red-crowned crane Grus japonensis in east Hokkaido, Japan (2015)
  • Author(s): Teraoka H, Okamoto E, Kudo M, Nakayama SMM, Ikenaka Y, Ishizuka M, Endo T, Kitazawa T, Hiraga T
  • Journal Title: Ecotoxicology and Environmental Safety Volume: 12 Pages: 11448-11465
  • DOI: 10.1016/j.ecoenv.2015.09.025
  • Peer Reviewed
• [Journal Article] Application of a Sediment Quality Index for the assessment and monitoring of metals and organochlorines in a premier conservation area (2015)
  • Author(s): Gerber R, Smit N, van Vuren J, Nakayama SMM, Yohannes YB, Ikenaka Y, Ishizuka M, Wepener V
  • Journal Title: Environ Sci Pollut Res Volume: 22 Pages: 19971-19989
  • DOI: 10.1007/s11356-015-5206-z
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Accumulation of heavy metals and metalloid from agricultural soils to foodstuffs around Tarkwa area in Ghana, and associated human health risks (2015)
  • Author(s): Bortey-Sam N*, Nakayama SMM*, Akoto O, Ikenaka Y, Fobil N.J., Baidoo E, Mizukawa H, Ishizuka M
  • Journal Title: Int J Environ Res Public Health Volume: 12 Pages: 8811-8827
  • DOI: 10.3390/ijerph120808811
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Cytochrome P450-mediated warfarin metabolic ability is not a critical determinant of warfarin sensitivity in avian species: In vitro assays in several birds and in vivo assays in chicken (2015)
  • Author(s): Watanabe KP, Kawata M, Ikenaka Y, Nakayama SMM, Ishii C, Darwish WS, Saengtienchai A, Mizukawa H, Ishizuka M
  • Journal Title: Environ Toxicol Chem Volume: 34 Pages: 2328-2334
  • DOI: 10.1002/etc.3062
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Uridine diphosphate-glucuronosyltransferase (UGT) xenobiotic metabolizing activity and genetic evolution in Pinniped species (2015)
  • Author(s): Kakehi M, Ikenaka Y, Nakayama SMM, Kawai YK, Watanabe KP, Mizukawa H, Nomiyama K, Tanabe S, Ishizuka M
  • Journal Title: Tox Sci Volume: 147 Pages: 360-369
  • DOI: 10.1093/toxsci/kfv144
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Health risk assessment of heavy metals and metalloid in drinking water from communities near gold mines in Tarkwa, Ghana (2015)
  • Author(s): Bortey-Sam N*, Nakayama SMM*, Ikenaka Y, Akoto O, Baidoo E, Mizukawa H, Ishizuka M
  • Journal Title: Environ Monit Assess Volume: 187 Pages: 1-12
  • DOI: 10.1007/s10661-015-4630-3
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Metabolic Activation of Heterocyclic Amines and Expression of Xenobiotic-Metabolizing Enzymes in the Gastrointestinal Tract of Rats (2015)
  • Author(s): Darwish WS, Nakayama SMM, Itotani Y, Ohno M, Ikenaka Y, Ishizuka M
  • Journal Title: J Food Sci Volume: 80 Pages: 1719-1726
  • DOI: 10.1111/1750-3841.12931
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Incidence and public health risk assessment of toxic metal residues (cadmium and lead) in Egyptian cattle and sheep meats (2015)
  • Author(s): Darwish WS, Hussein MA, El-Desoky KI, Ikenaka Y, Nakayama SMM, Mizukawa H, Ishizuka M
  • Journal Title: Int Food Res J Volume: 22 Pages: 1719-1726
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Metal extent in blood of livestock from Dandora dumping site, Kenya: source identification of Pb exposure by stable isotope analysis (2015)
  • Author(s): Nakata H*, Nakayama SMM*, Ikenaka Y, Mizukawa H, Ishii C, Yohannes YB, Konnai S, Darwish WS, Ishizuka M
  • Journal Title: Environ Pollut Volume: 205 Pages: 8-15
  • DOI: 10.1016/j.envpol.2015.05.003
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Characterization and tissue distribution of conjugated metabolites of pyrene in the rat (2015)
  • Author(s): Saengtienchai A, Ikenaka Y, Darwish WS, Nakayama SMM, Mizukawa H, Ishizuka M
  • Journal Title: J Vet Med Sci Volume: 77 Pages: 1261-1267
  • DOI: 10.1292/jvms.14-0632
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Are red gourami (Colisa labiosa) low xenobiotic metabolizers? Elucidation of in vivo pharmacokinetics of pyrene as a model substrate (2015)
  • Author(s): Ikenaka Y, Nakayama SMM, Oguri M, Saengtienchai A, Mizukawa H, Kobayashi J, Darwish WS, Ishizuka M
  • Journal Title: Environ Toxicol Pharmacol Volume: 39 Pages: 1148-1153
  • DOI: 10.1016/j.etap.2015.04.004
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Mercury concentrations in primary feathers reflect pollutant exposure in discrete non-breeding grounds used by Short-tailed Shearwaters (2015)
  • Author(s): Watanuki Y, Yamamoto T, Yamashita A, Ishii C, Ikenaka Y, Nakayama SMM, Ishizuka M, Suzuki Y, Niizuma Y, Meathrel CE, Phillips RA
  • Journal Title: Journal of Ornithology Volume: 156 Pages: 847-850
  • DOI: 10.1007/s10336-015-1205-6
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Levels, potential sources and human health risk of polycyclic aromatic hydrocarbons (PAHs) in particulate matter (PM10) in Kumasi-Ghana (2015)
  • Author(s): Bortey-Sam N, Ikenaka Y, Akoto O, Nakayama M.M.S, Yared YB, Baidoo E, Mizukawa H, Ishizuka M
  • Journal Title: Environ Sci Pollut Res Volume: 22 Pages: 1-10
  • DOI: 10.1007/s11356-014-4022-1
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Lead poisoning in children from townships in the vicinity of a lead-zinc mine in Kabwe, Zambia (2015)
  • Author(s): Yabe J*, Nakayama SMM*, Ikenaka Y, Yohannes YB, Bortey-Sam N, Oroszlany B, Muzandu K, Choongo K, Kabalo AN, Ntapisha J, Mweene A, Umemura T, Ishizuka M
  • Journal Title: Chemosphere Volume: 119 Pages: 941-947
  • DOI: 10.1016/j.chemosphere.2014.09.028
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Human health risks from metals and metalloid via consumption of food animals near Gold Mines in Tarkwa, Ghana: Estimation of the daily intakes and target hazard quotients (THQs) (2015)
  • Author(s): Bortey-Sam N*, Nakayama SMM*, Ikenaka Y, Akoto O, Yohannes YB, Baidoo E, Mizukawa H, Ishizuka M
  • Journal Title: Ecotoxicology and Environmental Safety Volume: 111 Pages: 160-167
  • DOI: 10.1016/j.ecoenv.2014.09.008
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Partial cloning of CYP2C23a genes and hepatic protein expression in eight representative avian species (2015)
  • Author(s): Watanabe KP, Kawai YK, Nakayama SMM, Ikenaka Y, Mizukawa H, Takaesu N, Ito M, Ikushiro S, Sakaki T, Ishizuka M
  • Journal Title: Journal of Veterinary Pharmacology and Therapeutics Volume: 38 Pages: 190-195
  • DOI: 10.1111/jvp.12159
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Identification of inter-specific differences in phase II reactions: Determination of metabolites in the urine of 16 mammalian species exposed to environmental pyrene (2015)
  • Author(s): Saengtienchai A, Ikenaka Y, Nakayama SMM, Mizukawa H, Kakehi M, Bortey-Sam N, Darwish W, Tsubota T, Terasaki M, Poapolathep A, Ishizuka M
  • Journal Title: Environmental Toxicology and Chemistry Volume: 33 Pages: 2062-2069
  • DOI: 10.1002/etc.2656
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 非実験動物における化学物質代謝能の特徴と種差 (2016)
  • Author(s): 池中良徳、筧麻友、水川葉月、中山翔太、石塚真由美
  • Organizer: 日本薬学会第136年会
  • Place of Presentation: パシフィコ横浜、神奈川
  • Year and Date: 2016-03-26 – 2016-03-29
  • Invited
• [Presentation] How to identify the high sensitive species to chemicals? ~Development of screening methods of phase II xenobiotic metabolism~ (2016)
  • Author(s): Yoshinori Ikenaka
  • Organizer: Second international veterinary medicine conference entitles: Role of veterinary medicine in development of animal wealth and human health safety
  • Place of Presentation: Suez, Egypt
  • Year and Date: 2016-03-15 – 2016-03-17
  • Int'l Joint Research / Invited
• [Presentation] 犬の慢性腸症における血中微量元素プロファイルの解析 (2016)
  • Author(s): 横山望, 大田寛, 水川葉月, 賀川由美子, 中山翔太, 佐々木東,森下啓太郎, 中村健介, 池中良徳, 石塚真由美, 滝口 満喜
  • Organizer: 第12回日本内科学アカデミー学術集会
  • Place of Presentation: パシフィコ横浜、神奈川
  • Year and Date: 2016-02-19 – 2016-02-21
• [Presentation] The habitat use of northern fur seals Callorhinus ursinus around northern Japan during non-breeding season, defined from stable isotopes and trace elements in whiskers (2015)
  • Author(s): Takanori Horimoto, Svetlana Artemyeva, Peter Permyakov, Mari Kobayashi, Kaoru Hattori, Hiromi Kasai, Hiroshige Tanaka, Yoshinori Ikenaka, Shouta M. M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka, Vladimir Burkanov, Russel Andrews, Yoko Mitani, Yasunori Sakurai
  • Organizer: 21st Biennial Conference on Marine Mammals
  • Place of Presentation: Hilton San Francisco Union Square, California, USA
  • Year and Date: 2015-12-13 – 2015-12-18
  • Int'l Joint Research
• [Presentation] Diet records in winter, migration and diving behavior during early summer of juvenile Steller sea lions in the asian stock (2015)
  • Author(s): Keizo Ito, Nao Takahashi, Kaoru Hattori, Yoko Goto, Takuya Nakanowatari, Kay I. Ohshima, Takanori Horimoto, Yoshinori Ikenaka, Shouta M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka, Kazushi Miyashita, Yoko Mitani
  • Organizer: 21st Biennial Conference on Marine Mammals
  • Place of Presentation: Hilton San Francisco Union Square, California, USA
  • Year and Date: 2015-12-13 – 2015-12-18
  • Int'l Joint Research
• [Presentation] Tracing migration of northern fur seals in Russian-Japanese waters using stable isotopes in whiskers (2015)
  • Author(s): Ayako Yamada, Takanori Horimoto, Yoshinori Ikenaka, Shouta M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka, Yoko Mitani, Kazushi Miyashita
  • Organizer: 21st Biennial Conference on Marine Mammals
  • Place of Presentation: Hilton San Francisco Union Square, California, USA
  • Year and Date: 2015-12-13 – 2015-12-18
  • Int'l Joint Research
• [Presentation] “バイオハザードコントロール”における“ケミカルハザード”～他分野連携における環境毒性学の重要性～ (2015)
  • Author(s): 池中良徳
  • Organizer: 環境ホルモン学会第18回研究発表会
  • Place of Presentation: 自治医科大学、栃木
  • Year and Date: 2015-12-10 – 2015-12-11
  • Invited
• [Presentation] Metabolism and biotransformation of organohalogen compounds in the liver microsomes of cats and dogs (2015)
  • Author(s): Hazuki Mizukawa, Kei Nomiyama, Nozomu, Yokoyama, Osamu Ichii, Mitsuyoshi Takiguchi, Yoshinori Ikenaka, Shouta M.M. Nakayama,Misaki Maehara, Shinsuke Tanabe, Mayumi Ishizuka
  • Organizer: SETAC North America 36th Annual Meeting
  • Place of Presentation: Salt Palace Convention Center, Salt Lake City, USA
  • Year and Date: 2015-11-01 – 2015-11-05
  • Int'l Joint Research
• [Presentation] Omics approach of proteomic and metabolomic analysis for PCBs toxicity in the dog brain (2015)
  • Author(s): Kohki Takaguchi, Kei Nomiyama, Lauan M. Claret, Momoko Kusaki, Jean Yoo, Hazuki Mizukawa, Hisato Iwata, Tatsuya Kunisue, Shinsuke Tanabe
  • Organizer: SETAC North America 36th Annual Meeting
  • Place of Presentation: Salt Palace Convention Center, Salt Lake City, USA
  • Year and Date: 2015-11-01 – 2015-11-05
  • Int'l Joint Research
• [Presentation] Contamination status of organohalogen compounds and their hydroxylated metabolites in pet cats: effects on thyroid hormones homeostasis (2015)
  • Author(s): Yasuo Yamamoto, Kei Nomiyama, Hazuki Mizukawa, Mitsuyoshi Takiguchi, Mayumi Ishizuka, Tatsuya Kunisue, Shinsuke Tanabe
  • Organizer: SETAC North America 36th Annual Meeting
  • Place of Presentation: Salt Palace Convention Center, Salt Lake City, USA
  • Year and Date: 2015-11-01 – 2015-11-05
  • Int'l Joint Research
• [Presentation] Phase II xenobiotic metabolizm in carnivora -UGT activity and genomestructure in Pinniped species- (2015)
  • Author(s): Yoshinori Ikenaka, Mayu Kakehi, Shouta Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
  • Organizer: 7th SETAC Africa Conference
  • Place of Presentation: Langebaan, South Africa
  • Year and Date: 2015-10-05 – 2015-10-08
  • Int'l Joint Research
• [Presentation] カブエ鉱床地域における鉛汚染とリモートセンシングを活用した今後の研究展望 (2015)
  • Author(s): 中山翔太
  • Organizer: 第五回超小型衛星に関する勉強会
  • Place of Presentation: 北海道大学東京オフィス、東京
  • Year and Date: 2015-09-09 – 2015-09-09
• [Presentation] 抗血液凝固系殺鼠剤の標的分子であるビタミンKエポキシド還元酵素(VKOR)の鳥類における特徴及び種差 (2015)
  • Author(s): 森田鮎子、池中良徳、中山翔太、石井千尋、水川葉月、渡邉研右、齊藤慶輔、渡邊有希子、伊藤真輝、高江洲昇、石塚真由美
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 北里大学獣医学部十和田キャンパス、青森
  • Year and Date: 2015-09-07 – 2015-09-09
• [Presentation] Interspecies differences in xenobiotic metabolism (2015)
  • Author(s): Mayumi Ishizuka
  • Organizer: 7th International Toxicology Symposium in Africa
  • Place of Presentation: Johannesburg, South Africa
  • Year and Date: 2015-08-31 – 2015-08-31
  • Int'l Joint Research / Invited
• [Presentation] Vector-control pesticide DDT detected in free-ranging chickens from South Africa (2015)
  • Author(s): Lesa A Thompson, Yoshinori Ikenaka, Yared B Yohannes, Johan van Vuren, Victor Wepener, Nico J Smit, Wynand Vlok, Shouta M M Nakayama, Hazuki Mizukawa and Mayumi Ishizuka
  • Organizer: 7th International Toxicology Symposium in Africa
  • Place of Presentation: Johannesburg, South Africa
  • Year and Date: 2015-08-31 – 2015-08-31
  • Int'l Joint Research / Invited
• [Presentation] Lead pollution in the children in Kabwe mining area, Republic of Zambia- Source identification by stable isotope analysis, 7th International Toxicology Symposium in Africa (2015)
  • Author(s): Shouta M M Nakayama, John Yabe, Yoshinori Ikenaka, Yared Beyene Yohannes, Balazs Oroszlany, Nesta Bortey-Sam, Kaampwe Muzandu, Hazuki Mizukawa, Kenedy Choongo, Abel Kabalo, Aaron Mweene, Mayumi Ishizuka
  • Organizer: 7th International Toxicology Symposium in Africa
  • Place of Presentation: Johannesburg, South Africa
  • Year and Date: 2015-08-31 – 2015-08-31
  • Int'l Joint Research / Invited
• [Presentation] Metallothionein as a biomarker for chicken exposure to heavy metals (2015)
  • Author(s): Wageh S. Darwish, Walaa F. Saad Eldin, Nesta Bortey-Sam, Yoshinori Ikenaka, Shouta M.M. Nakayama, Hazuki Mizukawa and Mayumi Ishizuka
  • Organizer: 7th International Toxicology Symposium in Africa
  • Place of Presentation: Johannesburg, South Africa
  • Year and Date: 2015-08-31 – 2015-08-31
  • Int'l Joint Research / Invited
• [Presentation] Histopathologic assessment of co-joint Cd and Pb exposure in rats (2015)
  • Author(s): John Yabe, Yoshinori Ikenaka, Shouta MM Nakayama, Wageh Sobhy Darwish, Nesta Bortey-Sam, Yared B. Yohannes, Aksorn Saengtienchai, Andy Saengtienchai, Takashi Umemura, and Mayumi Ishizuka
  • Organizer: 7th International Toxicology Symposium in Africa
  • Place of Presentation: Johannesburg, South Africa
  • Year and Date: 2015-08-31 – 2015-08-31
  • Int'l Joint Research / Invited
• [Presentation] Assessment and monitoring of drins from a premier conservation area (2015)
  • Author(s): Ruan Gerber, Nico J. Smit, Johan H.J. van Vuren, Yoshinori Ikenaka, Mayumi Ishizuka and Victor Wepener
  • Organizer: 7th International Toxicology Symposium in Africa
  • Place of Presentation: Johannesburg, South Africa
  • Year and Date: 2015-08-31 – 2015-08-31
  • Int'l Joint Research / Invited
• [Presentation] HCH’s in two fish species from a large floodplain pan within a subtropical conservation area (2015)
  • Author(s): Clare Edwards, Yoshinori Ikenaka, Yared Beyene, Shouta MM. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka, Victor Wepener, Johan .H.J. van Vuren
  • Organizer: 7th International Toxicology Symposium in Africa
  • Place of Presentation: Johannesburg, South Africa
  • Year and Date: 2015-08-31 – 2015-08-31
  • Int'l Joint Research / Invited
• [Presentation] Assessment of the food web structure of Xenopus muelleri from the lower Phongolo River floodplain using stable isotope analysis (2015)
  • Author(s): Nicolaas J. Wolmarans, Victor Wepener, Louis H. Du Preez, Yoshinori Ikenaka, Mayumi Ishizuka, Nico J. Smit
  • Organizer: 7th International Toxicology Symposium in Africa
  • Place of Presentation: Johannesburg, South Africa
  • Year and Date: 2015-08-31 – 2015-08-31
  • Int'l Joint Research / Invited
• [Presentation] Heavy metal residues in fish and shell fish marketed in Zagazig city, Egypt (2015)
  • Author(s): Mohamed A. Hussein, Wageh S. Darwish, Yoshinori Ikenaka and Mayumi Ishizuka
  • Organizer: 7th International Toxicology Symposium in Africa
  • Place of Presentation: Johannesburg, South Africa
  • Year and Date: 2015-08-31 – 2015-08-31
  • Int'l Joint Research / Invited
• [Presentation] Assessment of exposure to toxic elements (As, Pb, Cd and Hg), and DDTs in four species of birds from Ethiopia (2015)
  • Author(s): Yared Beyene Yohannes, Yoshinori Ikenaka, Shouta MM. Nakayama, Hazuki Mizukawa and Mayumi Ishizuka
  • Organizer: 7th International Toxicology Symposium in Africa
  • Place of Presentation: Johannesburg, South Africa
  • Year and Date: 2015-08-31 – 2015-08-31
  • Int'l Joint Research / Invited
• [Presentation] Exposure levels of polycyclic aromatic hydrocarbons (PAHs) and heavy metals in wild rats in Kumasi, Ghana (2015)
  • Author(s): Nesta Bortey-Sam, Yoshinori Ikenaka, Osei Akoto, Shouta M.M. Nakayama, Yared Beyene Yohannes, Elvis Baidoo, Aksorn Saengtienchai, Hazuki Mizukawa and Mayumi Ishizuka
  • Organizer: 7th International Toxicology Symposium in Africa
  • Place of Presentation: Johannesburg, South Africa
  • Year and Date: 2015-08-31 – 2015-08-31
  • Int'l Joint Research / Invited
• [Presentation] シンポジウム「野生鳥類の化学物質汚染とその影響」鳥類で起こっているケミカルハザードとそのメカニズム (2015)
  • Author(s): 中山翔太、水川葉月、池中良徳、石塚真由美
  • Organizer: 第21回日本野生動物医学会大会
  • Place of Presentation: 酪農学園大学、北海道
  • Year and Date: 2015-07-30 – 2015-08-02
• [Presentation] Lead poisoning and source identification in raptors from Japan (2015)
  • Author(s): Chihiro Ishii, Shouta M.M. Nakayama, Yoshinori Ikenaka, Hazuki Mizukawa, Yared B. Yohannes, Hokuto Nakata, Keisuke Saito, Yukiko Watanabe, Shinsuke Tanabe, Kei Nomiyama, Terutake Hayashi, Yasushi Masuda, Kentaro Q. Sakamoto, Mayumi Ishizuka
  • Organizer: Vth International Wildlife Management Congress 2015
  • Place of Presentation: Sapporo Convention Center, Sapporo, Japan
  • Year and Date: 2015-07-26 – 2015-07-30
  • Int'l Joint Research
• [Presentation] Vector-control pesticides disseminating via international waterways to accumulate in free-ranging freshwater and marine fish species (2015)
  • Author(s): Lesa A Thompson, Yoshinori Ikenaka, Johannes J van Vuren, Victor, Wepener, Nico Smit, Wynand Vlok, Yared B. Yohannes, Hazuki Mizukawa, Shouta M.M. Nakayama, Mayumi Ishizuka
  • Organizer: Vth International Wildlife Management Congress 2015
  • Place of Presentation: Sapporo Convention Center, Sapporo, Japan
  • Year and Date: 2015-07-26 – 2015-07-30
  • Int'l Joint Research
• [Presentation] Protective Effects of Carotenoids and Retinoids on Benzo[a]Pyrene Induced Mutagenicity and Oxidative Stress (2015)
  • Author(s): Wageh Sobhy Darwish, Yoshinori Ikenaka，Shouta M.M. Nakayama，Hazuki Mizukawa, Mayumi Ishizuka
  • Organizer: 42nd Annual Meeting of the Japanese Society of Toxicology
  • Place of Presentation: Kanazawa, Japan
  • Year and Date: 2015-06-29 – 2015-07-01
• [Presentation] Phase II酵素の欠損動物と非欠損動物―新たな動物種マッピング― (2015)
  • Author(s): 池中良徳, Aksorn Saengtienchai, 筧麻友, Wageh Sobhy Darwish, 水川葉月,中山翔太, 石塚真由美
  • Organizer: 第42回日本毒性学会
  • Place of Presentation: 金沢市、石川県
  • Year and Date: 2015-06-29 – 2015-07-01
  • Invited
• [Presentation] 猛禽類における鉛中毒問題と骨における鉛分布の解明 (2015)
  • Author(s): 石井千尋、中山翔太、池中良徳、水川葉月、中田北斗、森田鮎子、齊藤慶輔、渡邊有希子、神和夫、田辺信介、野見山桂、林光武、増田泰、坂本健太郎、石塚真由美
  • Organizer: 第24回環境化学討論会
  • Place of Presentation: 札幌コンベンションセンター、北海道
  • Year and Date: 2015-06-24 – 2015-06-26
• [Presentation] 野生齧歯類におけるワルファリン抵抗性獲得機序の解明 (2015)
  • Author(s): 武田一貴、池中良徳、田中和之、中山翔太、谷川力、水川葉月、石塚真由美
  • Organizer: 第24回環境化学討論会
  • Place of Presentation: 札幌コンベンションセンター、北海道
  • Year and Date: 2015-06-24 – 2015-06-26
• [Presentation] Concentrations of polycyclic aromatic hydrocarbon metabolites (OH-PAHs) in domestic animal in Kumasi, Ghana (2015)
  • Author(s): Nesta Bortey-Sam, Yoshinori Ikenaka, Osei Akoto, Shouta M.M. Nakayama, Jemima Marfo, Hazuki Mizukawa, Mayumi Ishizuka
  • Organizer: 24th Symposium on Environmental Chemistry
  • Place of Presentation: Sapporo Convention Center, Hokkaido, Japan
  • Year and Date: 2015-06-24 – 2015-06-26
  • Int'l Joint Research
• [Presentation] Nitrate content in Egyptian meat products and its modulatory effects on xenobiotic metabolizing enzymes (2015)
  • Author(s): Wageh Sobhy Darwish，Mohamed Abdallah Hussein, Yoshinori Ikenaka，Shouta M.M. Nakayama，Hazuki Mizukawa, Mayumi Ishizuka
  • Organizer: 24th Symposium on Environmental Chemistry
  • Place of Presentation: Sapporo Convention Center, Hokkaido, Japan
  • Year and Date: 2015-06-24 – 2015-06-26
  • Int'l Joint Research
• [Presentation] Insights into sub-tropical lowland river food webs using stable isotopes (2015)
  • Author(s): Ruan Gerber, Nico J. Smit, Johan H.J. Van Vuren, Shouta M.M. Nakayama, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: 24th Symposium on Environmental Chemistry
  • Place of Presentation: Sapporo Convention Center, Hokkaido, Japan
  • Year and Date: 2015-06-24 – 2015-06-26
  • Int'l Joint Research
• [Presentation] Vector-controlling pesticides in free-ranging chickens in KwaZulu-Natal, South Africa (2015)
  • Author(s): Lesa A Thompson, Yoshinori Ikenaka, Johannes J van Vuren, Victor, Wepener, Nico Smit, Wynand Vlok, Yared B Yohannes, Hazuki Mizukawa, Shouta M M. Nakayama, Mayumi Ishizuka
  • Organizer: 24th Symposium on Environmental Chemistry
  • Place of Presentation: Sapporo Convention Center, Hokkaido, Japan
  • Year and Date: 2015-06-24 – 2015-06-26
  • Int'l Joint Research
• [Presentation] Avian xenobiotic metabolizing genes: evolutionary relationships and mRNA expression in ostrich liver (2015)
  • Author(s): Yusuke K. Kawai, Kensuke P. Watanabe, Akihiro Ishii, Aiko Onuma, Hirofumi Sawa, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: 24th Symposium on Environmental Chemistry
  • Place of Presentation: Sapporo Convention Center, Hokkaido, Japan
  • Year and Date: 2015-06-24 – 2015-06-26
  • Int'l Joint Research
• [Presentation] 第II相抱合反応の種差：食肉目におけるグルクロン酸抱合酵素の進化と遺伝子構造 (2015)
  • Author(s): 池中良徳、筧麻友、中山翔太、川合佑典、渡邊研右、水川葉月、野見山桂、田辺信介、石塚真由美
  • Organizer: 第24回環境化学討論会
  • Place of Presentation: 札幌コンベンションセンター、北海道
  • Year and Date: 2015-06-24 – 2015-06-26
• [Presentation] ウマにおけるシトクロムP450の特性評価：ジアゼパム代謝を担うCYP3Aの重要性 (2015)
  • Author(s): 水川葉月、中山翔太、池中良徳、速水秋、Wageh Sobhy Darwish、渡邊研右、川合佑典、石塚真由美
  • Organizer: 第24回環境化学討論会
  • Place of Presentation: 札幌コンベンションセンター、北海道
  • Year and Date: 2015-06-24 – 2015-06-26
• [Presentation] タンチョウの水銀蓄積特性に関する検討 (2015)
  • Author(s): 寺岡宏樹、岡本絵梨佳、久道萌、中山翔太、池中良徳、石塚真由美、北澤多喜雄
  • Organizer: 第24回環境化学討論会
  • Place of Presentation: 札幌コンベンションセンター、北海道
  • Year and Date: 2015-06-24 – 2015-06-26
• [Presentation] 渡り性海鳥2種の越冬海域と羽の水銀濃度 (2015)
  • Author(s): 綿貫豊、山下愛、石塚真由美、池中良徳、中山翔太、石井千尋、山本誉士、伊藤元裕、桑江 朝比呂、鈴木裕也、新妻靖彰、C.E. Meathrel 、P.N. Trathan 、R.A. Phillips
  • Organizer: 第24回環境化学討論会
  • Place of Presentation: 札幌コンベンションセンター、北海道
  • Year and Date: 2015-06-24 – 2015-06-26
• [Presentation] Lead (Pb) poisoning in children from townships around an extensive lead-zinc mine in Kabwe, the Republic of Zambia (2015)
  • Author(s): Shouta M.M. Nakayama, John Yabe, Yoshinori Ikenaka, Yared Beyene, Nesta Bortey-Sam, Balazs Oroszlany, Kaampwe Muzandu, Kennedy Choongo, Aaron Mweene, Abel Kabalo, Hazuki Mizukawa, Mayumi Ishizuka
  • Organizer: 日本地球惑星科学連合2015年大会
  • Place of Presentation: 幕張メッセ、千葉
  • Year and Date: 2015-05-24 – 2015-05-28
• [Remarks] 北海道大学大学院獣医学研究科毒性学教室HP
  • URL: http://tox.vetmed.hokudai.ac.jp/
• [Funded Workshop] 7th Toxicology Symposium in Africa (2015)
  • Place of Presentation: ヨハネスブルグ、南アフリカ共和国
  • Year and Date: 2015-08-30 – 2015-09-01