Contribution of combined exposure to chemicals and ultraviolet rays in recent increase of skin cancer

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H02828
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: University of Shizuoka
• Principal Investigator: Ibuki Yuko 静岡県立大学, 食品栄養科学部, 教授 (30236781)
• Co-Investigator(Renkei-kenkyūsha): HIRAKAWA Kazutaka 静岡大学, 工学研究科 (60324513)
• Research Collaborator: ZHAO Xiaoxu ; YANG Guang
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 化学物質 / ヒストン / 紫外線 / 複合曝露 / NER / DNA損傷 / DNA修復 / たばこ

Outline of Final Research Achievements

In this study, the effect of exposure to chemicals on formation and repair of ultraviolet ray (UV)-induced DNA damag was examined in vitro and in vivo. In cultured human cells, cigarette sidestream smoke condensate (CSS), oxidant forms of polycyclic aromatic hydrocarbons, metal nanoparticles, etc. disrupted histone modifications, especially histone acetylation. In the condition of the disrupted histone modifications, the sensitivity to UV was increased. UV-induced DNA damage, pyrimidine dimers, were similarly formed, whereas the repair was delayed. Hairless mouse skin treated with CSS was exposed to UVB. The immunohistochemical staining of cyclobutane pyrimidine dimers revealed that their repair was delayed by the CSS treatment. These results suggested that combined exposure to some chemicals and UV may be a factor in increasing skin cancer.

Free Research Field

毒性学