2017 Fiscal Year Final Research Report
Development of variable-temperature high-speed AFM and its application to temperature-dependent ATPases
| Project/Area Number |
15H03540
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nanobioscience
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| Research Institution | Nagoya University (2017)
Kanazawa University (2015-2016) |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
IMADA KATSUMI 大阪大学, 理学研究科, 教授 (40346143)
YOKOYAMA KEN 京都産業大学, 総合生命科学部, 教授 (70271377)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 高速原子間力顕微鏡 / タンパク質 / 一分子計測 |
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| Outline of Final Research Achievements |
We have developed variable-temperature high-speed atomic force microscopy (VT-HS-AFM) with which the experimental temperature can be controlled ranging from a room temperature to around 40 ℃. The performance of VT-HS-AFM was confirmed by observation of fluidity of DPPC lipid bilayer with the phase-transition temperature of 41℃ from gel phase to liquid crystal phase. We applied the VT-HS-AFM system to observation of a flagellar protein, FliI, which is an ATPase with the activity optimum temperature over 40℃. HS-AFM images captured oligomerization processes of the FliI monomers to the hexamer and also conformational changes of the oligomers. Also we observed temperature dependent interaction between KaiC and KaiA which are proteins responsible to the circadian rhythm of Cyanobacteria.
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| Free Research Field |
生物物理学
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