Development of new techniques for cell regulation using synthetic self-localizing ligands

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H03835
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Nagoya Institute of Technology
• Principal Investigator: Tsukiji Shinya 名古屋工業大学, 工学(系)研究科(研究院), 教授 (40359659)
• Co-Investigator(Kenkyū-buntansha): 滝本 浩一 長岡技術科学大学, 工学研究科, 教授 (30500996)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 局在性リガンド / タンパク質局在 / オルガネラ / シグナル伝達 / 細胞機能制御

Outline of Final Research Achievements

The aim of this work was to develop new chemical biology techniques for controlling protein localization and cell signaling using synthetic self-localizing ligands (SLLs). We successfully developed new SLL-based methods that allow: (1) inducible protein translocation from the cytoplasm to the inner-leaflet of the plasma membrane, (2) orthogonal control of two distinct signaling molecules and pathways in single cells, (3) temporal control of signal duration, and (4) inducible protein translocation to the ER/Golgi membranes (endomembranes).

Free Research Field

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