2018 Fiscal Year Final Research Report
Nanoparticle Imaging with Vectorial Polarization Interferometric Laser Microscope
| Project/Area Number |
15H03945
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Intelligent mechanics/Mechanical systems
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| Research Institution | Shizuoka University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Keywords | レーザー顕微鏡 / 微粒子 / 偏光干渉 |
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| Outline of Final Research Achievements |
In recent years, applications of nano-particles to medical diagnosis and medical treatment have been studied intensively. Increasing attention to nanoparticle in particular has been devoted to drug delivery system (DDS). There are varieties of DDS technologies and a very significant number of DDS medicines already launched out in the world.
In this paper, incorporating a polarization interferometer into a nonlinear confocal microscope, we propose polarization-interferometric nonlinear confocal microscopy in order to analyze a single nanoparticle (200-nm diameter) doped with organic chromophores in liquid solutions. Measuring nanoparticles less than a half wavelength of an illuminating wave, general optical microscopes have poor spatial resolving power without toxic fluorescent indicator probes. We studied the contrast transfer function (CTF) and three dimensional spectroscopic imaging of the nanoparticle.
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| Free Research Field |
Nonlinear Optics
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| Academic Significance and Societal Importance of the Research Achievements |
現在のDDSはAFM表面計測が主流であり，より複雑な内部立体構造を有する球形デンドリマ等では計測が不可能で，より簡便で有効な構造解析手段が未だ提案されていないのが実情であり、各国がしのぎを削っている．当方が考案した偏光干渉顕微技術はこれをブレークスルーできる可能性があり，DDS輸送体の分光イメージングへ応用展開も可能であると判断し研究をスタートした．結果，提案技術はDDS輸送体の3次元分光計測において非常に有効な手法であり，医学・バイオの研究領域における波及効果が大きいということが確かめられた．
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