2017 Fiscal Year Final Research Report
Functional analysis of intergenic regions by contiguous introduction of genomic deletion mutations
| Project/Area Number |
15H04284
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Osaka University |
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Principal Investigator |
KOKUBU CHIKARA 大阪大学, 医学系研究科, 准教授 (70379238)
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| Research Collaborator |
TANAKA Sachiyo 大阪大学, 医学系研究科, 特任研究員
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | ゲノム工学 / 疾患モデル |
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| Outline of Final Research Achievements |
In order to enable effective functional analysis of genomic regions ranging from several hundred kilobases to several megabases, this study was focused on development of a new method by combining conventional genome engineering and editing technologies.LoxP elements were transposed in the mouse embryonic stem cell genome, followed by simultaneous induction of site-specific Cre-mediated recombination and CRISPR/Cas-mediated editing.This approach allowed introduction of the intended long-range rearrangements into the genome, but the introduction efficiency has not been significantly improved. The positional combinations of the LoxP sites and CRISPR targets remain to be further optimized.
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| Free Research Field |
遺伝学
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