Establishment of heredital desease models using iPS cells and genome editing

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04286
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Kyoto University (2017); University of Miyazaki (2015-2016)
• Principal Investigator: Honda Arata 京都大学, 京都市, 特定准教授 (10373367)
• Co-Investigator(Kenkyū-buntansha): 下島 圭子 東京女子医科大学, 医学部, 特任助教 (30578935) ; 山海 直 国立研究開発法人医薬基盤・健康・栄養研究所, 医薬基盤研究所 霊長類医科学研究センター, 主任研究員 (80300937)
• Co-Investigator(Renkei-kenkyūsha): OGURA Atsuo 独立行政法人理化学研究所, バイオリソース研究センター, 室長 (20194524) ; MATSUDA Osamu 京都府立医科大学, 医学系研究科, 教授 (00271164)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: Experimental Animals / ES cells / iPS cells / naive / germ cells / chimera

Outline of Final Research Achievements

This research project had been aimed for the establishment of heredital desease models using iPS cells and genome editing using several animal species. We achieved naive-like conversion of cynomolgus monkey ES/iPS cells, which can enhances their in vitro differentiation potential. Moreover, following mice and rats, the 3rd true naive-iPS cells were successfully established form an endangered species, Tokudaia osimensis. We produced interspecific chimera and elucidated the sexual plasticity of the germ cells of the endangered species.

Free Research Field

Experimental Animals