Functional analysis of novel oncogene dynAP and the identification of its inhibitors

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04314
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Tumor therapeutics
• Research Institution: Nagahama Institute of Bio-Science and Technology
• Principal Investigator: Mizukami Tamio 長浜バイオ大学, バイオサイエンス学部, 教授 (80367896)
• Co-Investigator(Renkei-kenkyūsha): SASAKI Ryuzo 長浜バイオ大学, バイオサイエンス学部, 客員教授 (60077378) ; HASEGAWA Makoto 長浜バイオ大学, バイオサイエンス学部, 教授 (10367899) ; NAKAMURA Toshinobu 長浜バイオ大学, バイオサイエンス学部, 教授 (80403202) ; NAGAI Nobuo 長浜バイオ大学, バイオサイエンス学部, 教授 (90260281) ; TAKAHASHI Rei 同志社女子大学, 薬学部, 教授 (60144565)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 分子標的治療

Outline of Final Research Achievements

Human dynactin-associated protein (dynAP) is a transmembrane protein that promotes AktSer473 phosphorylation. NIH3T3 cells expressing dynAP vigorously formed spheroids in anchorage-deficient three-dimensional culture. NIH3T3dynAP cells injected into nude mice produced tumors with abundant blood vessels and weak cell-cell contacts. Thus, dynAP could be a new oncoprotein and a target for cancer therapy. Here, we investigate the biological properties of dynAP including structural and functional analysis of the sugar chains in dynAP and identified lead antibodies and chemicals targeting dynAP. These findings would promote dynAP targeting drug development.

Free Research Field

がん分子標的治療