Rescue of 21 trisomy in Down syndrome cells using spindle assembly checkpoint

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04321
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical genome science
• Research Institution: Hiroshima University
• Principal Investigator: Matsuura Shinya 広島大学, 原爆放射線医科学研究所, 教授 (90274133)
• Co-Investigator(Kenkyū-buntansha): 宮本 達雄 広島大学, 原爆放射線医科学研究所, 講師 (40452627) ; 柳原 啓見 広島大学, 原爆放射線医科学研究所, 助教 (50719474)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 染色体 / 細胞周期チェックポイント

Outline of Final Research Achievements

The aim of this study was to normalize 21 trisomy in Down's syndrome cells by induction of chromosome instability. We transfected BubR1 siRNA into Down's syndrome cells and analyzed their karyotypes. Cellular phenotype of mosaic variegated aneuploidy was observed, but a cell clone in which excessive chromosome 21 was normalized was not yet obtained. We will establish such cell clones that lost only excessive chromosome 21 by increasing the number of isolated cell clones.

Free Research Field

人類遺伝学