Mechanism of selective substrate cleqvage and novel cellular functions ofintramembrane protease RseP

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04350
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Kyoto University
• Principal Investigator: Akiyama Yoshinori 京都大学, ウイルス・再生医科学研究所, 教授 (10192460)
• Co-Investigator(Kenkyū-buntansha): 森 博幸 京都大学, ウイルス・再生医科学研究所, 准教授 (10243271)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 生体膜 / プロテアーゼ / 膜内切断 / 表層ストレス応答 / 膜タンパク質

Outline of Final Research Achievements

Intramembrane cleaving proteases (I-CLiPs) are known to act in a variety of important cellular processes in diverse organism. RseP, an Escherichia coli S2P family intramembrane cleaving protease, is involved in regulation of the extracytoplasmic stress response and membrane quality control through specific cleavage of substrates within the lipid bilayer. We have shown that, in addition to the periplasmic PDZ domain that acts as a size-exclusion filter, the cytoplasmic MRE β-loop region and the C1N region play critical roles in specific recognition and substrate cleavage.

Free Research Field

分子生物学