Study of protein-assemble/disassemble on biological membrane depending on the membrane shape by high-speed AFM

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04360
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Kanazawa University
• Principal Investigator: Kodera Noriyuki 金沢大学, 数物科学系, 教授 (30584635)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 一分子イメージング / 高速AFM / 生体膜 / 膜タンパク質 / 生体分子

Outline of Final Research Achievements

Biological membranes have uneven physical shapes. By recognizing on the physical shapes, lipids and proteins specifically assemble and disassemble, and realize their sophisticated functions. In this research project, to realize the real-time observations of these biological processes by high-speed AFM, new AFM substrates with sub-micrometer sized holes have been developed on a glass and PDMS surfaces using Focused Ion Beam (FIB) lithography and nanosphere imprinting. Using the AFM substrates developed here, some phenomena and objects depending on the physical shapes of membrane, diffusion of lipid molecules, assembling of a membrane protein as well as a suspended native membrane, were directly visualized.

Free Research Field

生物物理学