General mechanisms controlling development of cerebellar neural circuits in vertebrates

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04376
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Nagoya University
• Principal Investigator: HIBI MASAHIKO 名古屋大学, 生物機能開発利用研究センター, 教授 (40273627)
• Co-Investigator(Renkei-kenkyūsha): TAKEUCHI MIKI 名古屋大学, 生物機能開発利用研究センター, 研究員 (60625127)
• Research Collaborator: SHIMIZU TAKASHI 名古屋大学, 生物機能開発利用研究センター, 准教授 (50324760)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 小脳 / 神経回路形成 / ゼブラフィッシュ / 神経発生 / 神経分化 / 層形成 / 転写因子 / 可視化

Outline of Final Research Achievements

Using zebrafish as a main model system, (1) we isolated granule cell- and Purkinje cell-specific genes by RNA-seq. We found that (2) Contactin1b is involved in neural circuit formation for motor learning and motor coordination; (3) Reeling signaling is involved in migration of Purkinje cells in the cerebellum and type I neurons in the optic tectum, Reelin is transported through granule cell axons; (4) the transcription factors Ptf1a and Gsx2 are required for differentiation of neurons in the inferior olive nuclei, the expression of gsx2 is regulated by retinoic acid signaling; (5) the transcription factors Fop1b/4 and the transcriptional co-repressors Skor1b/2 function as master regulators in differentiation of Purkinje cells.

Free Research Field

発生生物学