Real-time imaging analysis of highly ordered structure of cell wall in trees

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04527
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Wood science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Funada Ryo 東京農工大学, (連合)農学研究科(研究院), 教授 (20192734)
• Co-Investigator(Kenkyū-buntansha): 梶田 真也 東京農工大学, (連合)農学研究科(研究院), 教授 (40323753) ; 半 智史 東京農工大学, (連合)農学研究科(研究院), 准教授 (40627709) ; 渡邊 宇外 千葉工業大学, 先進工学部, 准教授 (70337707)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 木質バイオマス / 細胞壁 / 管状要素 / 微小管 / 培養細胞 / リグニン / 植物ホルモン / 生体イメージング

Outline of Final Research Achievements

For the highly utilization of wood biomass, we need to understand the mechanism of differentiation of secondary xylem in trees such as the process of formation of highly ordered structure of cell wall. We developed new in vitro model system that induced directly secondary xylem-like tracheary elements from cultured cells of trees such as hybrid poplar and some conifers. Tracheary elements formed highly developed structure of secondary walls, such as broad regions of thick cell walls, bordered pits and helical thicknenings. Using this model system, we analyzed the pattern of lignification in cell walls and cell corners by histochemical techniques. In addition, we analyzed the localization of microtubules using stable expression of genes for microtubule-associated proteins with green fluorescent protein. The microtubules play an important role in the determination of localization of secondary wall.

Free Research Field

木質科学