Development of biological marker for breeding goldfish strain resistant to herpesviral hematopoietic necrosis

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04544
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Aquatic bioproduction science
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: Sano Motohiko 東京海洋大学, 学術研究院, 教授 (00372053)
• Co-Investigator(Kenkyū-buntansha): 坂本 崇 東京海洋大学, 学術研究院, 教授 (40313390)
• Research Collaborator: TANAKA Mikio
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 水産学 / 病理学 / ウイルス / 耐病機構 / 選抜育種マーカー

Outline of Final Research Achievements

We investigated mechanisms of resistance against herpesviral hematopoietic necrosis (HVHN) in a resistant strain of goldfish and tried to develop biomarkers utilizing in selection breeding without virus infection.
Virus susceptibility of primary cell culture derived from fin of the resistant fish is significantly lower than that of sensitive strain. In resistant fish, virus load increased for 3 days after virus infection like in the sensitive fish and subsequently it drastically decreased. MHC class I gene expression was significantly higher in the resistant fish than in the sensitive fish before and after virus infection. The resistant trait showed Mendelian dominant inheritance. Genotyping-by-sequencing (GBS) method was applied to exploration of single nucleotide polymorphisms (SNP) and the linkage analysis of the SNPs showed that two SNPs were significantly related with the resistance to HVHN. Finally, we developed a qPCR for MHC I gene expression and HRM for the SNP detection.

Free Research Field

水族病理学