Activation of protoplast function by nanobubbles and development of high-speed production system for useful metabolites

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04569
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Agricultural environmental engineering/Agricultural information engineering
• Research Institution: University of Tsukuba
• Principal Investigator: AOYAGI Hideki 筑波大学, 生命環境系, 教授 (00251025)
• Co-Investigator(Renkei-kenkyūsha): BAMBA Takeshi 九州大学, 生体防御医学研究所, 教授 (10432444) ; NAKAO Yoichi 早稲田大学, 理工学術院, 教授 (60282696) ; OU Hekisyou 流通経済大学, 経済学部, 教授 (80261775)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ナノバブル / エチレン / プロトプラスト / バイオリアクター / 追熟 / 植物 / 微生物 / 細胞壁

Outline of Final Research Achievements

The nanobubble generation system was originally developed. This system can generate nanobubbles of various gases with high efficiency. The generated nanobubbles could stably exist in different solutions and media. Various investigations using yeast, plants cells and protoplasts suggested that the metabolites biosynthesized by protoplasts were released into the culture broth, with the double benefits of increasing the overall productivity and facilitating downstream processing. Various nanobubbles enhance those phenomena. Application of nanobubbles to yeast, various plants cells and protoplasts resulted in efficient production of useful metabolites. The bioreactor capable of supplying nanobubbles was also developed. The nanobubbles can be also used for ripening of fruit, inactivation of microbes, removal of chemicals on fruit surface, etc.

Free Research Field

生物化学工学、細胞機能開発工学