Osteochondral regeneration using allogenic implantation of somatic stem cell

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04600
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Kagoshima University
• Principal Investigator: MISUMI Kazuhiro 鹿児島大学, 農水産獣医学域獣医学系, 教授 (10291551)
• Co-Investigator(Kenkyū-buntansha): 須永 隆文 鹿児島大学, 農水産獣医学域獣医学系, 助教 (90649112) ; 中山 功一 佐賀大学, 医学部, 特任教授 (50420609) ; 瀬戸山 健太郎 鹿児島大学, 学内共同利用施設等, 准教授 (00372805) ; 藤木 誠 鹿児島大学, 農水産獣医学域獣医学系, 准教授 (60305167)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 幹細胞 / 軟骨 / 骨 / 移植 / 再生

Outline of Final Research Achievements

Regeneration of articular cartilage and subchondral bone using scaffold-free constructs composed of adipose tissue-derived mesenchymal stem cells (AT-MSCs) using bio three-dimensional (3D) printer was evaluated in minipigs. Osteochondral defects created in medial chondyles of femurs were implanted by autologous construct (autologous implantation), allogenic construct (allogenic implantation), and no construct (controls). Post-surgical computed tomography demonstrated the delayed regeneration of subchondral bone in allogenic implantation, since the radiolucent volume of defects significantly decreased in the autologous implantation comparing to allogenic implantation. Also, total scores of either the magnetic resonance image or histopathology were significantly higher in the autologous implantation than the allogenic implantation. Allogenic implantation of scaffold-free 3D-constructs of AT-MSCs could delay the regeneration in osteochondral defect comparing to autologous implantation.

Free Research Field

獣医外科学