2018 Fiscal Year Final Research Report
Molecular basis of recycling and quality control of chloroplasts by selective autophagy
| Project/Area Number |
15H04626
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
牧野 周 東北大学, 農学研究科, 教授 (70181617)
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| Research Collaborator |
IZUMI Masanori
WADA Shinya
EGUCHI Masatake
HIROTA Takaaki
YOKOHAMA Ryo
NISHIMURA Tsubasa
NAKAMURA Sakuya
ISHIDA Hiromi
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Keywords | オートファジー / 葉緑体 / シロイヌナズナ / 栄養リサイクル |
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| Outline of Final Research Achievements |
We aimed to reveal molecular mechanisms of selective autophagy of chloroplasts and obtained following results. (1) We revealed roles of known ATG8-interacting proteins, NBR1, ATG11, and ATI1/2 on the RCB (Rubisco-containing body) pathway and chlorophagy (whole-chloroplast autophagy). (2) We isolated novel mutants having abnormality on the RCB pathway. (3) We searched novel autophagy receptors which may act for chloroplast by co-immunoprecipitation and LC-MS/MS analysis.
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| Free Research Field |
植物栄養生理学
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| Academic Significance and Societal Importance of the Research Achievements |
植物に特有のオルガネラである葉緑体に注目することで、選択的オートファジーのメカニズムについて酵母や動物では得られない基礎的知見を提供した。オートファジーによる葉緑体のリサイクルや品質管理機構は、必須栄養素の体内転流、光合成、バイオマスや有用物質生産などと密接にかかわっている。その分子機構の一端を解明したことは、今後の作物の生産性や品質の向上を目指す農学研究の展開に向けても意義深い。
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