2017 Fiscal Year Final Research Report
Development of a novel fluorescent labeling method for detecting heterooligomers of membrane proteins on living cells
| Project/Area Number |
15H04636
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
河野 健一 京都大学, 化学研究所, 助教 (70732874)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 蛍光イメージング / 膜タンパク質 |
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| Outline of Final Research Achievements |
New live-cell labeling methods were examined to detect GPCR hetero-oligomers in combination with the conventional coiled-coil labeling method. 1) β-hairpin and 2) RNA aptamer labeling principles were not successful because of the insufficient expression of tagged receptors on the cell surface. 3) Collagen heterodimer probe peptides and collagen-tagged GPCR were also prepared to examine the expression of the tagged receptors and selective labeling.
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| Free Research Field |
生物物理化学
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