Regulation of epigenetic enzymes via nitrosative stress and development of its specific modulator

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04649
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pharmacology in pharmacy
• Research Institution: Okayama University
• Principal Investigator: Uehara Takashi 岡山大学, 医歯薬学総合研究科, 教授 (00261321)
• Co-Investigator(Kenkyū-buntansha): 伊藤 昭博 東京薬科大学, 生命科学部, 教授 (40391859)
• Co-Investigator(Renkei-kenkyūsha): 永坂 岳司 岡山大学, 大学院医歯薬学総合研究科, 講師 (30452569)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 一酸化窒素 / Sーニトロシル化 / 炎症反応 / 遺伝子発現 / エピゲノム / 病態形成

Outline of Final Research Achievements

We could demonstrate that nitric oxide (NO) regulates epigenetic enzymes. A cysteine residue was a target of NO and its covalent modification contributed to the inhibition of enzymatic activity in cells. From these findings, we speculated that NO may be involved in the up-regulation of several genes via this mechanism. Next, we conducted DNA micro array for isolating genes that is sensitive to NO. We could isolate several genes regulated by NO. Thus, we could propose a novel system of gene expression via nitrosative stress in this study.

Free Research Field

薬理学