Regenerative mechanisms of pancreatic endocrine and exocrine cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04812
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Keio University
• Principal Investigator: KO SHIGERU 慶應義塾大学, 医学部(信濃町), 准教授 (90402578)
• Co-Investigator(Kenkyū-buntansha): 石黒 啓一郎 熊本大学, 発生医学研究所, 准教授 (30508114) ; 竹田 直樹 熊本大学, 生命資源研究・支援センター, 助教 (90304998)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 膵内外分泌細胞 / 細胞分化誘導 / ヒトES細胞

Outline of Final Research Achievements

The aim of this study was to develop GLP1 receptor knock-in mice and to develop a method for inducing β cell differentiation from human pluripotent stem cells.Recombination vector was introduced into mouse ES cells to establish genetically modified mice. Pancreatic endocrine cells from these mice showed no fluorescence.Transcription factors were introduced into human ES cells by the lipofection method. Expression of pancreatic endocrine hormone such as insulin was confirmed as early as two weeks after induction of differentiation. A method to induce cell differentiation from human ES cells to pancreatic endocrine cells was established.

Free Research Field

膵再生医学