Stemness and tumorigenesis of salivary gland myoepithelial cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H05013
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Showa University
• Principal Investigator: Mishima Kenji 昭和大学, 歯学部, 教授 (50275343)
• Co-Investigator(Kenkyū-buntansha): 入江 太朗 岩手医科大学, 歯学部, 教授 (00317570) ; 安原 理佳 昭和大学, 歯学部, 講師 (20453649) ; 田中 準一 昭和大学, 歯学部, 助教 (40710166) ; 馬渕 洋 東京医科歯科大学, 大学院保健衛生学研究科, 助教 (50424172) ; 斎藤 一郎 鶴見大学, 歯学部, 教授 (60147634) ; 桐田 忠昭 奈良県立医科大学, 医学部, 教授 (70201465) ; 阪井 丘芳 大阪大学, 歯学研究科, 教授 (90379082) ; 福島 美和子 昭和大学, 歯学部, 助教 (90548273) ; 河野 葉子 昭和大学, 歯学部, 准教授 (40195681)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 唾液腺 / 再生 / 筋上皮細胞 / 幹細胞

Outline of Final Research Achievements

We attempted to identify stem/progenitor cells in salivary gland. Localization of BrdU label-retaining cells were found specifically in myoepthelial cells located between luminal and stroma cells after 30 weeks, suggesting that myoepithelial cells were expected to be one of putative stem cells. The data from RNA-seq showed CD49fhigh subset contained positive cells for both of αSMA and Myh11, a marker of myoepithelial cells, demonstrating that CD49fhigh subset consisted of myoepithelial cell-enriched population. The selection of a CD49fhigh subset showed high ability of sphere formation and was differentiated into ductal lineage in vitro. These data suggest that the enriched myoepithelial cell compartment may contain stem/progenitor cells in salivary glands since the cells enriched in CD49fhigh subset showed multipotent properties by sphere formation.

Free Research Field

口腔病理