Development of biomarker for IgAN: Development of biomarker for IgAN: An international collaboration study

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H05292
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Kidney internal medicine
• Research Institution: Fujita Health University
• Principal Investigator: Yuzawa Yukio 藤田保健衛生大学, 医学部, 教授 (00191479)
• Co-Investigator(Kenkyū-buntansha): 比企 能之 藤田保健衛生大学, 医学部, 教授 (20156566) ; 秋山 真一 名古屋大学, 医学系研究科, 特任講師 (20500010) ; 長谷川 みどり 藤田保健衛生大学, 医学部, 教授 (40298518) ; 高橋 和男 藤田保健衛生大学, 医学部, 講師 (90631391)
• Research Collaborator: Jan Novak University of Alabama at Birmingham, 教授 ; Renfrow Matthew B. University of Alabama at Birmingham, 准教授 ; Francesco Paolo Schena University of Bari, 教授
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: IgA腎症 / 糖鎖異常IgA / バイオマーカー開発 / 国際間共同研究 / 糖鎖医学 / 人種差 / 翻訳後修飾解析

Outline of Final Research Achievements

IgA1 with galactose (Gal)-deficient hinge-region (HR) O-glycans (Gd-IgA1) plays a key role in the pathogenesis of IgA nephropathy (IgAN). To identify potentially disease-specific IgA1 HR O-glycoforms, we profiled serum IgA1 HR glycopeptides using specimens from Japanese IgAN patients and healthy controls (HC) of different races. IgA1 from sera of 20 Japanese IgAN patients and 50 HC, recruited from Caucasian, Black, Hispanic, Asian, and Japanese populations, was purified by affinity chromatography. IgA1 HR glycosylation heterogeneity including glycan attachment sites were analyzed by liquid chromatography-high-resolution mass spectrometry. We successfully characterized HR O-glycan profile in normal subjects and identified the O-glycoforms correlating with serum level of Gd-IgA1. Identification of specific HR O-glycoforms increasing in IgAN patients will lead to the development of robust biomarker in IgAN.

Free Research Field

腎臓内科学