2016 Fiscal Year Final Research Report
A strategy for the treatments of refractory neuropathic pain using Schwann-like cells derived from dermal fibroblasts
| Project/Area Number |
15H06040
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Anesthesiology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Keywords | シュワン細胞 / ミエリン / 神経障害性痛 |
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| Outline of Final Research Achievements |
We induced Schwann-like cells by an existing method with minor modifications.
To estimate myelination, which is indispensable to nerve regeneration, we put the induced cells into artificial tubes, transplanted them between the cut ends of rat's sciatic nerve, observed the contents in the grafts. Myelin formation was successfully observed by electron microscopy and immunofluorescent staining.
Existing neuropathic pain models have the problems of remaining threads and are not suitable for the research for the purpose of cell transplantation therapies. Thus, we developed a new rat model with clips. The model rats are free from remaining artifacts, showed the drop of pain threshold and microglial proliferation, that are the characteristics of neuropathic pain.
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| Free Research Field |
神経再生
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