Making an Aligned Muscle Cell Embedded Collagen Gel Sheet on a Parallel Patterned Calcium Alginate Substrate

2015 Fiscal Year Final Research Report

• Project/Area Number: 15H06074
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Pediatric surgery
• Research Institution: University of Tsukuba
• Principal Investigator: HAGIWARA Koki 筑波大学, 医学医療系, 研究員 (80761650)
• Project Period (FY): 2015-08-28 – 2016-03-31
• Keywords: 再生医療 / 骨格筋再生 / 組織再生工学 / 小児外科 / 筋シート / アルギン酸カルシウム

Outline of Final Research Achievements

This study's purpose was to make an aligned muscle cell sheet and its substrate to regenerate skeletal muscle through sheet transplantation. As a result, the creation of a calcium alginate substrate with aligned 400um diameter half column ditches and an aligned myotube embedded collagen gel sheet were a success. The ditches made aligned patterns in the gel; and the non adhesive feature of the substrate sheet let the proteins and cells contract smoothly on one axis. Also, double layer muscle cell sheets sandwiching the substrate were made to handle the sheet for easy transplantation. In addition, calcium alginate honeycomb meshes were made for the sheet transplantation. The dried mesh held sheets and transplanted to the target area easily. The mesh could then be removed or sutured in to interrupt other tissue attachment. Last, a tough collagen sheet sandwiched by cell embedded collagen gels was made for transplantation into areas needing high mechanical strength, such as the diaphragm.

Free Research Field

再生医療