Development of LC-MS/MS-based accurate quantification methods for assessing serum vitamin D metabolites

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H06096
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Laboratory medicine
• Research Institution: Chiba University
• Principal Investigator: ISHIGE Takayuki 千葉大学, 医学部附属病院, 臨床検査技師 (30757315)
• Research Collaborator: SATOH Mamoru 千葉大学, 医学部附属病院, 特任准教授 (20401002) ; NOMURA Fumio 千葉大学, 医学部附属病院, 特任教授 (80164739) ; NISHIMURA Motoi 千葉大学, 医学部附属病院, 講師 (80400969) ; OGAWA Shoujiro 東京理科大学, 薬学部, 助教 (30546271) ; HIGASHI Tatsuya 東京理科大学, 薬学部, 教授 (90272963)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: ビタミンD代謝物 / LC-MS/MS / 誘導体化

Outline of Final Research Achievements

DAPTAD derivatization of vitamin D metabolites were performed for enhancing the ionization efficiency of LC-MS/MS. As compared with 25(OH)D3, the intensity of 25(OH)D3-DAPTAD was increased approximately 100-fold higher. By using SLE for sample preparation, the high-sensitive and robust method for quantification of 4 serum vitamin D metabolites was established. We are planning to establish serum 1,25(OH)2D analysis method by LC/MS/MS.

Free Research Field

病態検査学