Intracellular ATP- and redox potential-responsive siRNA delivery carrier

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H06108
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: The University of Tokyo
• Principal Investigator: NAITO MITSURU 東京大学, 大学院医学系研究科(医学部), 特任研究員 (50755329)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: siRNA / 環境応答性 / 高分子ミセル / 還元環境 / ATP

Outline of Final Research Achievements

To enhance an efficacy of cancer cell specific siRNA delivery, novel siRNA-loaded polyion complex micelles with intracellular-responsive siRNA releasability has been developed. A cyclic peptide which can selectively bind to cancer cell membrane proteins was introduced onto PIC micellar surface to enhance the targetability. A phenylbotronic acid and thiol moiety were introduced into PIC micellar core to increase PIC micellar stability at extracellular condition and release siRNA into cytoplasm in response to intracellular condition. The newly prepared stimuli-responsive PIC micelles did release siRNA under intracellular mimic condition.

Free Research Field

DDS