Research of oxidative stress tolerance mechanism in head and neck cancer using glutathione-sensitive fluorescent probes

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H06175
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Otorhinolaryngology
• Research Institution: The University of Tokyo
• Principal Investigator: Yoshida Masafumi 東京大学, 医学部附属病院, 講師 (80396754)
• Research Collaborator: URANO Yasuteru
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: 頭頸部癌 / 酸化ストレス / 蛍光イメージング

Outline of Final Research Achievements

We developed fluorescent probe targeted for glutathione (GSH). To overcome the problems of existing probes, we developed the FRET type fluorescent probe, nameed QuicGSH series, using a reversible fluorescence control mechanism. In addition to the quantification of GSH in cultured cells , new findings on oxidative stress tolerance mechanism were achieved. Based on the findings obtained in this study, further elucidate the resistance to treatment of cancer cells and application for cancer therapy are expected.

Free Research Field

頭頸部外科学