2016 Fiscal Year Final Research Report
Establishment of a highly efficient culture method for mesenchymal stem cells based on cell adhesion-dependent cell cycle changes
| Project/Area Number |
15H06177
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Keywords | 再生医療 / 培養 / 組織 / 浮遊細胞 / トランスレーショナルリサーチ / 歯学 |
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| Outline of Final Research Achievements |
In this study, we examined protocols for the digestion of articular and auricular cartilages and determined the optimal conditions for articular cartilage digestion. The maximum numbers of viable cells were obtained after digestion in 0.15, 0.3, or 0.6% collagenase for 24 h, in 1.2% collagenase for 6 h, or in 2.4% collagenase for 4 h. In tissues incubated in 0.15 or 0.3% collagenase, even at 24 h, possibly reflecting incomplete digestion of cartilage. Cell damage appeared to be greater when collagenase concentrations were high. We recommend a 24-h incubation in 0.6% collagenase as the optimal condition for chondrocyte isolation from articular cartilage. Moreover, we found that the optimum cell-seeding density is approximately 3,000 cells/cm2. Conditions determined in this study would maximize the yield of isolated articular chondrocytes and enable the generation of a large quantity of cultured cells.
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| Free Research Field |
歯学
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