2016 Fiscal Year Annual Research Report
Quantitative analysis of virus particles using nanopore-based resistive pulse sensing technique
| Project/Area Number |
15H06205
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
楊 路 東京工業大学, 工学院, 研究員 (60757392)
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| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Keywords | Biosensing / Virus particle / Resistive pulse sensing |
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| Outline of Annual Research Achievements |
This study aims to develop an efficient sensing method to realize label-free, long-term and continuous monitoring of virus particles that are widespread in our surrounding environment. Recently, Tunable Resistive Pulse Sensing (TRPS) technology has emerged as a promising tool to quantify biomolecules and nanoparticles. TRPS counts particles by detecting the transient change in resistance of a nanopore when particles translocate across it one by one.
The physical properties of baculovirus and adenovirus were measured using TRPS technology. First, the particle size distributions (PSD) were obtained from the magnitude of resistive pulse signals. This PSD information can be used to determine if the particles aggregated. Then, the total particle concentrations were determined according to the translocation frequency of the particle. Additionally, the infectious titer of adenovirus was carried out to obtain the ratio of total to infective virus particles, which is an important parameter indicating the infectivity level of viruses. Finally, the zeta potential that has been inadequate was also measured from the translocation rate of the particle. Our results were in good agreement with other studies, demonstrating the feasibility of TRPS. Moreover, the bivariate histogram showed that it is possible to discriminate between different viruses. These preliminary results enabled us to access the basic and in-depth characteristics of various virus particles. In the future, measuring as many viruses as possible and fabricating our own resistive pulse sensor will be our next step.
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| Research Progress Status |
28年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
28年度が最終年度であるため、記入しない。
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Research Products
(2 results)
