2016 Fiscal Year Final Research Report
Development of thermo-responsive magnetic conjugates designed for isolation and purification of circulating tumor cells
Project/Area Number |
15H06260
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Single-year Grants |
Research Field |
Nanomaterials engineering
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Research Institution | Nagoya Institute of Technology |
Principal Investigator |
Fuchigami Teruaki 名古屋工業大学, 工学(系)研究科(研究院), 助教 (20756704)
|
Project Period (FY) |
2015-08-28 – 2017-03-31
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Keywords | 磁性ナノ粒子 / 磁気細胞分離 / 血中循環腫瘍細胞 / 温度応答性高分子 / がん早期診断 |
Outline of Final Research Achievements |
Novel thermo-responsive magnetic conjugates composed of iron oxide particles, streptavidin and thermos-responsive polymer were developed to achieve high-efficiency enrichment and purification of circulating tumor cells (CTCs) with no stress. Controlling an amount of streptavidin on a surface of the conjugates, capture efficiency of CTCs increased from 25 to 60%. Observing the conjugates motion on lung cancer cells under microscope, the thermo-responsive conjugates captured and released cancer cells by changing temperature from body temperature (37°C) to lower temperatures. According to these results, the novel thermos-responsive conjugates has an advantageous for the high-efficiency enrichment and the purification.
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Free Research Field |
nanotechnology
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